Field-amplified sample stacking (FASS) leverages conductivity gradients between a volume of injected sample and the background buffer to increase sample concentration. A major challenge in applying FASS to on-chip assays is the initial setup of high-conductivity gradient boundaries in the region of the injected sample volume. We have designed, fabricated, and characterized a novel FASS-capillar...

A miniaturized analytical system for separating and detecting toxic organophosphate nerve agent compounds, based on the coupling of a micromachined capillary electrophoresis chip with a thick-film amperometric detector, is described. Factors influencing the on-chip separation and detection processes have been optimized. Using a MES buffer (20 mM, pH 5.0) running buffer, a 72-mm-long separation ...

Various separation processes have been integrated in microfluidics, such as capillary electrophoresis and chromatography, on a microchip. However, it is extremely difficult to separate a complicated biological system by conventional methods. Here, we report on a feasible structure and the culture condition of human renal proximal tubule epithelial cells (RPTECs), with the aim to construct a bio...

A common approach in resolving enantiomers of chiral basic drugs by capillary electrophoresis (CE) is to use cyclodextrins (especially their anionic derivatives) as chiral selector in the acidic buffer (pH ≤ 3) in normal or reversed (carrier) mode. Then, some organic modifiers are added to the buffer solution if the resolution is not satisfactory. In case of cetirizine (CTN), applying the same ...

Capillary electrophoresis was used lbr the qualitative as well as quantitative analysis of phosphopeptides. Three pairs of phosphorylated peptides and non-phosphorylated analogues were analyzed by capillary electrophoresis. Each sequence of phosphopeptides contained one phosphoserine, phosphothreonine or phosphotyrosine residue. The capillary electrophoresis analyses were performed in a fused-s...

This paper presents a miniaturized bioreactor (bioreactor-on-a-chip) applied to baker’s yeast fermentation. The bioreactor-on-a-chip is fabricated using a silicon and glass wafers applying micromachining technology (wet-etching techniques) in order to create microchannels, mixerchannels, microvalves. The miniaturization and integration allows smaller volumes to be used, which can be often rathe...