Identification of individual component from a natural mixture sample is having the utmost importance in the drug development industry. Generally, plant components or natural material on the earth use to present in a complex form and the individual components have been found to have specific beneficiary effect on specific disease. Such urgency to isolate the unit component or active ingredient i...

A novel strain, Microbacterium sp., which can be used as a catalyst in the asymmetric reduction of simple aromatic ketones to produce chiral aromatic alcohols, was isolated from soil by microbial screening. Using acetophenone as a model substrate for asymmetric reduction, 82 % reaction yield with more than 99 % enantiomeric excess of (R)-1-phenylethanol were achieved under the optimal condition...

Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLC-based methods. Some traditional applications of this approach include the us...

Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLC-based methods. Some traditional applications of this approach include the us...

Abstract Background Direct measurement of the bioavailable α-tocopherol content presents a significant analytical challenge and requires chiral separation stereoisomers. Objective The objective study was to validate an method for analysis stereoisomers in infant formulas dairy products. Method Samples were saponified at elevated temperature lipophilic components extracted into organic solvent, ...