A sensitive radioisotope microassay has been developed for the estimation of cholesteryl ester hydrolase (EC 3.1.1.13) in preparations of isolated rabbit aortic smooth muscle cells. Kinetic studies served to establish optimal assay conditions, which involve incubation in a total volume of 0.2 ml containing 5 pM cholesteryl oleate tritiated in the cholesterol moiety, 0.4 mM egg yolk lecithin, 0....

We examined the roles of phospholipase A2 (PLA2) in oxidized LDL (oxLDL)-induced cholesteryl ester formation in macrophages. In [3H]oleic acid-labeled RAW264.7 cells and mouse peritoneal macrophages, oxLDL induced [3H]cholesteryl oleate formation with an increase in free [3H]oleic acid and a decrease in [3H]phosphatidylcholine. The changes in these lipids were suppressed by methyl arachidonyl f...

Chylomicrons containing labeled cholesterol, mainly (70%) present as cholesteryl ester, were injected intravenously into intact rats, and samples of liver were obtained 27-210 min later . Most (58-75%) of the injected label was recovered in the liver after 27-75 min . Hepatic uptake occurred without hydrolysis of the labeled cholesteryl ester . In separate experiments, in vitro perfusion of liv...

Preeclampsia is accompanied by an extensive remodeling of the extracellular matrix of umbilical cord. It is associated with an increase in collagen content in the umbilical cord artery. Furthermore, preeclampsia distinctly reduces proteolytic and gelatinolytic activity, especially after activation with various agents.We decided to develop a method for separation and determination of fatty acids...

Previously, we prepared cationic nanoparticles (NP and NP-N) composed of cholesteryl diamine (OH-Chol, (3S)-N-(2-(2-hydroxyethylamino)ethyl)cholesteryl-3-carboxamide) and cholesteryl triamine (OH-N-Chol, (3S)-N-(2-(2-(2-hydroxyethylamino)ethylamino)ethyl)cholesteryl-3-carboxamide), respectively, with Tween 80 for small interfering RNA (siRNA) delivery into tumor cells. In this study, we prepare...

Introduction: Recent studies suggest that dietary virgin olive oil (VOO) reduces hypoxia-re oxygenation injury in rat brain. We have attempted to determine the effect of dietary virgin olive oil on brain lipidomics and its relationship with brain edema in a rat stroke model. Methods: Five groups, each consisting of 6 male Wistar rats, were studied. The first and second groups (control and s...

In previous papers (1,2) the thermotropic properties and the kinetics of the bulk polymerization of the mesomorphic monomer cholesteryl acrylate have been described. In addition to cholesteryl acrylate, the methacrylate was prepared, as well as the respective acrylic methacrylic acid ester of cholestanol. We now wish to report the properties of these monomeric esters and the results of their po...

White Carneau (WC) pigeons are susceptible to the development of aortic atherosclerosis, whereas Show Racer (SR) pigeons are resistant, even though there are no differences in the known risk factors, including plasma cholesterol levels, lipoproteins, blood pressure, etc. Although this suggests that the difference in atherosclerosis susceptibility between WC and SR pigeons may be mediated at the...

Fatty acids are transported to cells from a variety of different moieties in the plasma. In this study, using oleate and human umbilical vein endothelial cells, we asked whether the vehicle that delivers fatty acid to cells has an influence on its metabolism upon its incorporation into the cell. For oleate vehicles, we compared free oleate bound to albumin with oleate in low density lipoprotein...