Interactions of three new isophorone derivatives, Isoa Isob and Isoc with salmon testes DNA have been investigated using UV-Vis, fluorescence and circular dichroism spectroscopic methods. All the studied compounds interact with DNA through intercalative binding mode. The stoichiometry of the isophorone/DNA adducts was found to be 1:1. The fluorescence quenching data revealed a binding interacti...

In vitro chlorophyll (Chl) aggregates have often served as models for in vivo forms of long-wavelength Chl. However, the interaction of protein-bound Chl molecules is typically different than that occurring in solvent-based self-aggregates. We have chosen a water-soluble Chl-binding protein (WSCP) from cauliflower in order to help characterize the spectroscopic properties of Chl in a single wel...

Circularly-polarized extreme ultraviolet and X-ray radiation is useful for analysing the structural, electronic and magnetic properties of materials. To date, such radiation has only been available at large-scale X-ray facilities such as synchrotrons. Here, we demonstrate the first bright, phase-matched, extreme ultraviolet circularly-polarized high harmonics source. The harmonics are emitted w...

Circular dichroism spectroscopy has been extended to the VUV region through the introduction of the photo-elastic modulator with a calcium fluoride optical element. Spectroscopic and stereochemical applications of the technique to chiral molecules in the vapour and in the condensed phase are reviewed, together with related linear dichroism studies of orientated molecular systems. 1 . Introducti...

Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy are analytical techniques employed for the analysis of protein secondary structure. The use of CD spectroscopy is limited to low protein concentrations (<2 mg ml(-1)), while FTIR spectroscopy is commonly used in a higher concentration range (>5 mg ml(-1)). Here we introduce a quantum cascade laser (QCL)-based IR transmis...

MOTIVATION Circular dichroism (CD) spectroscopy is one of the most versatile tools to study protein folding and to validate the proper fold of purified proteins. Here, we aim to provide a readily accessible, user-friendly and platform-independent tool capable of analysing multiple CD datasets of virtually any format and returning results as high-quality graphical output to the user. RESULTS C...

Circular dichroism (CD) is useful in molecular chemistry, pharmaceuticals, and bio-sensing. In this paper, twisted Z-shaped nanostructure (TZN) is proposed to achieve giant CD. The TZN is composed of three vertical and twisted nanorods. Given that the resonance of vertical nanorod is only observable for left circularly polarized light excitation but is subdued for right circularly polarized lig...

We propose a Babinet-invertible chiral metasurface for achieving dynamically reversible and strong circular dichroism (CD). The proposed is composed of VO$_2$-metal hybrid structure, when VO$_2$ transits between the dielectric state metallic state, unit cell switches complementary structures that are designed according to Babinet principle. This leads large CD tuning range $\pm 0.5$ at 0.97~THz...

Protein interactions with surfactants are dependent on their physiochemical properties. The effect of cationic gemini surfactant hexanediyl-a,u-bis-(N-(2-hydroxyethyl)-N-methylhexadecylammonium dibromide) on the stability and activity of hen egg white lysozyme was compared with its monomeric counterpart N-(2-hyroxyethyl)-N,N-dimethylhexadecylammonium bromide at pre and post micellar concentrati...

Matrix absorption and magnetic circular dichroism studies on 0s160, and OS’804 have been carried out to clarify the number of electronic transitions present in the region. The spectra show that the irregular vibrational structure in the I60 species with its onset at -276 nm is perfectly regular in the “0 compound. The system is, therefore, interpreted as a single electronic system analogous to ...