نتایج جستجو برای: cloning efficiency
تعداد نتایج: 450287 فیلتر نتایج به سال:
A new cloning strategy is described which utilizes direct selection of recombinants for shotgun sequencing in the filamentous bacteriophage M13. Direct selection is accomplished by insertional inactivation of the M13 gene X protein, a powerful inhibitor of phage-specific DNA synthesis when overproduced. An extra copy of gene X was inserted into the intergenic region of M13 and placed under the ...
Reverse genetics has become pivotal in influenza virus research relying on rapid generation of tailored recombinant influenza viruses. They are rescued from transfected plasmids encoding the eight influenza virus gene segments, which have been cloned using restriction endonucleases and DNA ligation. However, suitable restriction cleavage sites often are not available. Here, we describe a clonin...
The efficacies of guide RNAs (gRNAs), the short RNA molecules that bind to and determine the sequence specificity of the Streptococcus pyogenes Cas9 nuclease, to mediate DNA cleavage vary dramatically. Thus, the selection of appropriate target sites, and hence spacer sequence, is critical for most applications. Here, we describe a simple, unparalleled method for experimentally pre-testing the e...
a highly efficient cloning vector was constructed for cloning pcr products by inserting an 80 bp dna fragment into pgem-5zf (+) vector. the xcm i digestion of this vector gave rise to a 3’ overhanging deoxythymidine offering the possibility of cloning pcr products with 3' adenosine overhang created by taq dna polymerase. furthermore, two ecor i sites were added to the construct for identificati...
مقدمه: بروز نتایج متفاوت در آزمایشگاهها به علت استاندارد نبودن، از معایب روشهای تکثیر مولکولی است. در بیشتر مقالات چاپ شده در زمینه تشخیص pcr عوامل عفونی، اینترنال کنترل تکثیری (ic) وجود ندارد. هدف از پژوهش حاضر، طراحی و توسعه یک روش ساده و سریع، برای ساخت اینترنال کنترل آزمون pcr در آزمایشگاههای تشخیصی است. مواد و روشها: در پژوهش حاضر، برای ساخت اینترنال کنترل رقابتی، از پ...
background: tuberculosis (tb) remains as a major cause of death around the world. construction of a new vaccine against tuberculosis is an effective way to control it. several vaccines against this disease have been developed. the aim of the present study was to cloning of tb10.4 gene in pcdna3.1+ plasmid and evaluation of its expression in eukaryotic cells. methods: firstly, tb10.4 fragment wa...
Animal cloning is one of the animal biotechnology branches. Although this method has been used since 1950, but many have paid attention to cloning by birth of Dally. Because the way of Dolly production was completely different from other existed methods in cloning.This new-found and unprecedented method has been called "Somatic Cell Nuclear Transfer" (SCNT), so scientists have eagerly paid atte...
cloning is a term traditionally used to describe different processes for duplicating biological material. a clone is an organism that is a genetic copy of an existing one. the use of the technique of nuclear transfer for reproduction of human beings is surrounded by strong ethical and legal concerns and controversies. some international organizations have made significant efforts to establish c...
Religious positions on human cloning are pluralistic in modes of argument and conclusions. Some religious thinkers argue that cloning a human is immoral and propose a ban on human cloning. Some other religious thinkers contend that human cloning could be morally justified under some circumstances. The Roman Catholic Church expressed a vigorous opposition to the procedure and a Vatican editor...
BACKGROUND Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian cell types with high efficiency, regardless of proliferation state. Furthermore, the use of inducible promoters to drive shRNA expression allows for more thorough investigation...
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