AIM The present study was carried out on semen ejaculates of 15 Karan Fries (KF) bulls maintained at Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, India with an objective to evaluate the relationship between the conventional and fluorescent based semen quality analysis of the bulls. MATERIALS AND METHODS A total of 96 ejaculates were collected from 15 KF (Hol...

this is the first report implementing combined density gradient centrifugation/zeta (dgc/zeta) sperm selection procedure for a couple with eleven previous intra cytoplasmic sperm injection/ in vitro fertilization (icsi/ivf) failure cycles. semen analysis was carried out according to world health organization criteria. protamine deficiency, dna fragmentation and morphology were assessed by chrom...

background: intrauterine insemination (iui) is one of the therapeutic approaches for infertility. the objective of this study was to evaluate dna integrity and apoptosis role in success of iui in both mild male and female factor infertility. methods: patients were divided into two groups: m (mild male factor; n=29) and f (female factor; n=31) undergoing single iui. ejaculates were analyzed and ...

The family Rhamphichthyidae includes three genera: Rhamphichthys Müller et Troschel, 1846, Gymnorhamphichthys M. M. Ellis, 1912 and Iracema Triques, 1996. From this family, only the species Rhamphichthys hanni Meinken, 1937 has had its karyotype described. Here, we describe the karyotypes of two additional Rhamphichthys species: Rhamphichthys marmoratus Castelnau, 1855 from the Reserva de Desen...

Background: The aim of this study was to examine the possible relationship between sperm DNA integrity and chromatin packaging evaluated by cytochemical assays, traditional sperm parameters and recurrent spontaneous abortion (RSA) of unknown origin. Materials and Methods: In this cohort study, 40 couples with a history of RSA and 40 couples with proven fertility were considered as case and cont...

Background: Intracytoplasmic sperm injection (ICSI) can be considered the solstice in vitro insemination technique because it has powerfully allowed the treatment of male factor infertility, but in this procedure, except visual morphological selection, there is no standardization for sperm selection. Recently, hypo osmotic swelling test (HOST) has been proposed to have the potential to select i...

To determine the protective effects of exogenous melatonin following exposure to Bleomycin, Etoposide, and Cisplatin (BEP) chemotherapy on sperm parameters DNA integrity, testes nitro-oxidative status, as well histopathological, inflammatory, apoptotic alternations in rats testes. Adult male Wistar were randomly segregated into six groups (n=10/group). Groups 1, 3, 4 intraperitoneally injected ...

Ancistrus Kner, 1854 is a diverse catfish genus, currently comprising 66 valid species, but karyotype data were recorded for 33 species, although only ten have their taxonomic status defined. Considerable karyotype diversity has been found within this genus, with 2n varying from 34 to 54 and structural variability including heteromorphic sex chromosomes. In many cases, uncertainty on the taxono...