The paper presents this new therapeutical method, its. application fields, hypothesis regarding the acting mechanism at the infracellular, cellular and systemic level, the practical methodology, and some personal results. We finish the paper with general proposals for standardization of the scientific works from this field, which should be necessary due to a large variety of approaching to this...

HE SEPARATION of protein from nonprotein material, a usual procedure in the analysis of biological specimens, has been effected in a ilumber of ways. Marymont and London (1) have reported a new coagulalation technic which is perhaps advantageous in certain instances, as well as several applications of the technic (2-6). it is well known that the precipitation of proteins by any of the conventio...

A 75-kDa protein secreted from mouse coagulating gland was purified to homogeneity by a series of isolation steps including ion exchange chromatography on a DEAE-Sephacel column and ion exchange high-performance liquid chromatography on a sulfopropyl column. It was identified to be Type IV transglutaminase (TG(4)), based on the establishment of N-terminal sequences by automated Edman degradatio...

Seminal vesicle secretory protein IV (SVS IV) is perhaps the most abundant protein made by the epithelial cells of the rat seminal vesicle. In this report, we have asked whether the methylation of its gene correlates with its tissue-specific pattern of expression. Using methylation-sensitive restriction endonucleases HpaII and AvaI, we could examine seven potential methylation sites in or near ...

THE mitogenic actions of hormonal materials have been studied in a variety of tissues belonging to the endocrine system proper or to its “target tissues”. The results of many such studies have been tabulated by Eigsti and Dustin [ 141. Estrogens and androgens have been demonstrated to be powerful mitotic stimulators of both the stromal tissues and epithelia of such glands as the seminal vesicle...

Studies inquiring into the mechanism of blood clotting have been made by many careful investigators, and while much is written regarding the factors concerned, there is offered as yet no satisfactory and uniformly accepted explanation for t.he fact that blood usually clots when it escapes from a wound. Some of this confusion exists, perhaps, because the more recent investigations in colloidal c...