This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system. The multi-step screen involves a first round using a 2-dimensional, cell-line-based scrape-healing assay, then a second round using a 3-dimensional tubulogenesis assay; both of these rounds use new cell lines described in this report. The fi...

Linked Open University Data applies semantic web and linked data technology to university data scenario, aiming at building interlinked semantic data around university information, providing possibility for unified innerand interschool information query and comparison. This paper proposes a general process of building linked open university data, with procedures covering choosing datasets and v...

We pioneer a new future in robotic dust collection by introducing passive dust-collecting robots that, unlike their predecessors, do not require locomotion to collect dust. While previous research has exclusively focused on active dust-collecting robots, we show that these robots fail with respect to practical and theoretical aspects, as well as human factors. By contrast, passive robots, throu...

Acute renal injury induces metabolic acidosis, but its specific effects on the collecting duct, the primary site for urinary ammonia secretion, the primary component of net acid excretion, are incompletely understood. We induced ischemia-reperfusion (I/R) acute renal injury in Sprague-Dawley rats by clamping the renal pedicles bilaterally for 30 min followed by reperfusion for 6 h. Control rats...

The mammalian kidney collecting duct plays an important role in the fine regulation of Na, K, water, and acid-base balance. Functional genomic and proteomic studies of the kidney offer new opportunities in the understanding of renal physiology and pathophysiology, and the collecting duct is an appropriate target tissue because of the relative simplicity of its cells and the ease of isolating or...

The effects of vasopressin, angiotensin II, and calcium ionophore A23187 were examined and compared on endogenous prostaglandin E2 (PGE2) production, in the absence of exogenous arachidonate, by glomeruli and cortical and medullary collecting tubules freshly prepared from rat kidney. Angiotensin II stimulated PGE2 production in the glomeruli but not in the collecting tubules. Vasopressin at con...

Patients value the interpersonal aspects of their health care experiences. However, faced with multiple resource demands, primary care practices may question the value of collecting and acting upon survey data that measure patients' experiences of care. The Consumer Assessment of Healthcare Providers and Systems (CAHPS) suite of surveys and quality improvement tools supports the systematic coll...

Mobile, touch-screen devices are increasingly ubiquitous in children’s lives. The extensive use of such devices presents an exciting opportunity for data collection. We describe a simple method for creating cross-platform, interactive tablet experiments using open Web-based resources. We illustrate this method by collecting data from 1to 4-year-old children in a word-recognition paradigm, using...

We have three-dimensionally reconstructed the lymphatic architecture of the rabbit tongue using a computer graphic three-dimensional reconstruction method together with histochemically stained serial cryo-sections. Three collecting lymphatic vessels lying in an anteroposterior orientation were identified in the tongue body. A superior longitudinal muscle accompanying collecting lymphatic (SLCL)...