In this study, anion-exchange chromatography was used to purify factor VII and factor IX from prothrombin complex (PPSB), which contains coagulation factors II, VII, IX and X. For this purpose, DEAE-Sepharose CL-6B gel , Pharmacia column XK-26 , high flow rate and two stepwise gradients with phosphate citrate buffer were used. The yield of the two lyophylized products, factor VII and factor...

Several biotinyl estradiol derivatives have been prepared by coupling estradiol 7 alpha-carboxylic acid to biotin via different linear linkers. All these compounds exhibit a high affinity for the estrogen receptor as determined by competitive binding assays against [3H]estradiol. These compounds also displaced the dye 4-hydroxyazobenzene-2'-carboxylic acid from the biotin-binding sites of avidi...

We compared and statistically evaluated the effectiveness of nine DNA extraction procedures by using frozen and dried samples of two silt loam soils and a silt loam wetland sediment with different organic matter contents. The effects of different chemical extractants (sodium dodecyl sulfate [SDS], chloroform, phenol, Chelex 100, and guanadinium isothiocyanate), different physical disruption met...

Optimizing or debottlenecking existing production plants is a challenging task. In this case study, an reversed phased chromatography polishing step for peptide purification was optimized with the help of digital twin. The batch depicted digitally general rate model. Model parameter determination and model validation done dedicated experiments. twin then used to identify process variants, espec...

A method for the determination of phosphorylation sites in phosphoproteins based on column-switching high-performance liquid chromatography (HPLC) has been developed. The HPLC system consisted of a titania precolumn for the selective adsorption of phosphopeptides, an anion-exchange analytical column and a UV detector (215 nm). Rabbit muscle phosphorylase a (RPa) and porcine stomach pepsin (PSP)...

Since the introduction of catalysts and reagents on solid-support, researchers have developed new reaction systems to take advantage of their insoluble nature by designing multistep reaction sequences, high-throughput purification techniques, and combinatorial synthesis methods. The continuous flow system is one of these advancements and represents the foundation of a new technique termed seque...

Twenty-one fungal species were screened for capability of removing pyrene and benzo(a)pyrene in culture. Xylaria regalis was found to show the highest activity. An PAHs-degrading enzyme was purified to homogeneity from the culture mycelium of X. regalis. The crude enzyme was subjected to a series of purification procedures, including QAE Sephadex A-25 anion exchange column, hydrophobic interact...

Background and objectives: The aerial parts of Echinophora platyloba are added to cheese and yoghurt for flavoring. Besides, it is used for the treatment of flatulence and as air freshener. Despite several pharmacological studies on the plant, no attempt has been made to isolate non-volatile secondary metabolites from this plant. So, phytochemical investigation seems t...

Supplement 1: Methods Purification procedure of tissue kallikrein Rat kallikrein was purified from urine to apparent homogeneity by ultrafiltration and ion-exchange chromatography on a DEAE-Sepharose FF column (GE Healthcare, Buckinghamshire, UK), affinity chromatography on an aprotinin (Sigma, MO, USA) coupled HiTrap NHS-activated Sepharose HP column (GE Healthcare) and gel filtration twice on...

A novel alkaline phosphatase (S-ALP) was found in the culture filtrate of Streptomyces hiroshimensis IFO 12785. Purification was achieved on Sephadex G-75 column, palmitoylated gauze column, and Superdex 75 HR column chromatographies. The molecular weight of S-ALP was estimated to be 14200 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The isoelectric point is 9.2. S-A...