نتایج جستجو برای: competitive elisa test
تعداد نتایج: 931681 فیلتر نتایج به سال:
Lumpy skin disease (LSD) is a notifiable, transboundary cattle that spreads rapidly and has relevant economic impact. The etiological agent the LSD virus (LSDV), genus Capripoxvirus, family Poxviridae. To date, LSDV widely present in Africa, Asia, transcontinental regions like Russia, Turkey, Middle East, thus representing continuous threat to free neighbours. Appropriate serosurveillance progr...
Background and Aim: The affinity of an antibody with its antigen is a crucial parameter in its biological activity and carrying out immunologic tests such as radioimmunoassay (RIA) and immunohistochemistry. This study was done in our laboratory at Tarbiat Modarres University to measure the affinity constant of specific monoclonal antibodies/MAB (A1G8F7) with alkaline phosphatase enzyme produced...
Main indoor allergens for humans are from house dust mites. There are more than 30 allergens in Dermatophagoides farinae but only fourteen allergens have been identified from this mite including Der f 1-3, 6, 7, 10, 11, 13-18, and 22. A native allergen protein (Der f 24, 90 kDa) was purified from D. farinae by gel filtration and anionic exchange liquid chromatography combined with IgE immunodet...
BACKGROUND Most insulin autoantibody assays for both human and animal models are in a radioassay format utilizing (125)I-insulin, but despite the radioassay format international workshops have documented difficulty in standardization between laboratories. There is thus a need for simpler assay formats that do not utilize radioactivity, yet retain the high specificity and sensitivity of radioass...
Adducts of benzo[a]pyrene-diolepoxide (BPDE) with blood nucleophiles have been used as biomarkers of exposure to polycyclic aromatic hydrocarbons (PAHs). The most popular such assay is a competitive enzyme-linked immunosorbent assay (ELISA) that employs monoclonal antibody 8E11 to detect benzo[a]pyrene tetrols following hydrolysis of BPDE adducts from lymphocyte DNA or human serum albumin (HSA)...
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