Individual plant growth-promoting rhizobacterial (PGPR) strains (Bacillus thuringiensis B2, Bacillus subtilis B10 and Enterobacter cloacae B16) their combination were used, with or without Pythium oligandrum, against Sclerotinia sclerotiorum. In vitro, all biological control agents (BCAs) reduced successfully hyphal growth of the targeted pathogenic fungus. They displayed antifungal activity by...

Single-strand conformational polymorphism analysis of an affected male with Pelizaeus-Merzbacher disease (PMD) showed a slight change in mobility of amplified exon 5 of the proteolipid protein (PLP) gene. The exon was sequenced and a G-->A transition at codon 216 was found. This mutation eliminates a BstNI restriction site and creates a MaeI restriction site. In 1989, Gencic et al. reported a m...

Cowden syndrome is an autosomal dominant condition of multiple hamartomas. Patients with this phakomatosis have an increased risk of breast cancer and thyroid tumours. Lhermitte-Duclos disease is usually a sporadic condition of cerebellar ganglion cell hypertrophy, ataxia, mental retardation, and self-limited seizure disorder. We describe a three generation family with Cowden syndrome and Lherm...

We present a practical application of polymerase chain reaction single-strand conformational polymorphism (PCR-SSCP) using a fluorescence-based image analyzer. Fluorescence-labeled primers were used to amplify target sequences. After nondenaturing polyacrylamide gel electrophoresis using a conventional sequencing gel apparatus, the glass plate was directly transferred from the gel box to the im...

We have examined melanocytic cells derived directly from fresh biopsy tissue for the presence of p53 mutations. Using selective media that permits growth of melanocytes and inhibits growth of fibroblasts and keratinocytes, we established short-term, primary cultures of melanocytes from skin biopsies of common acquired nevi, dysplastic nevi, and from metastatic melanoma. Using PCR-single-strande...