•Gap junction protein Innexin2 modulates adult Drosophila activity rhythms•Innexin2 is expressed and functions in the ventral lateral neurons•Innexin2 levels influence PERIOD oscillations•Innexin2 affects amplitude of cycling PDF s-LNv neuronal terminals A circuit ∼150 neurons rhythmic activity-rest behavior melanogaster. While it known that coherent ∼24-hr rhythms locomotion are brought about ...