"Reproductive" cloning and "therapeutic" or "research" are both deliberate attempts to create humans that genetically identical. Human reproductive in general is prohibited by a number of international regional agreements, including the Charter Fukushima, Additional Protocol Council Europe Convention on Rights Biomedicine, World Health Organization resolutions implications for human health, Uni...

Background: Tuberculosis (TB) remains as a major cause of death around the world. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning of tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryotic cells. ...

In the context of quantum information there arise a number of interesting questions regarding the domains of operations which are feasible in classical context to be applicable in quantum information domain or not. The criterion of feasibility and non feasibility of various physical processes in quantum regime creates a set of impossible operations[1-6]. This may be probably due to the linear s...

BACKGROUND Designing an ideal vaccine against HIV-1 has been difficult due to enormous genetic variability as a result of high replication rate and lack of proofreading activity of reverse transcriptase leading to emergence of genetic variants and recombinants. Tat transactivates HIV-1 LTR, resulting in a remarkable increase in viral gene expression, and plays a vital role in pathogenesis. The ...

It is expected that more accounting conservation (environmental uncertainty) reduces manager optimism. Prior research, however, has struggled to establish this relation empirically. Moreover, some evidence points to the possibility that the manager optimism is lower for firms with more accounting conservation. In this paper, the author examine the link between accounting conservation, environme...

We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation. Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day. We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, especially for long inserts above 2.5 kb. EMP furth...

UNLABELLED This research focused on understanding the attitudes toward human cloning in Taiwan among professionals in healthcare, law, and religion. DESIGN The study was conducted utilizing a structured questionnaire. PARTICIPANTS 220 healthcare professionals from two regional hospitals located in Taipei, 351 religious professionals in the northern Taiwan and 711 legal professionals were se...

Abstract At present, many cloning selection algorithms have been studied, and improvements made to the cloning, mutation steps. However, there is a lack of research on optimization updating operation The clonal algorithm traditionally updated through random complement antibodies, which blind uncertain process. added antibodies may gather near local optimal solution, resulting in need for more i...