PURPOSE To determine the safety of prophylactic intracameral moxifloxacin 0.5% ophthalmic solution (Vigamox) in patients having cataract surgery. SETTING American Eye Center, Manila, Philippines. METHODS Preoperative and 1-month postoperative anterior chamber reaction, corneal endothelial cell density, and corneal thickness were assessed in 65 eyes that had cataract surgery with intracamera...

Basement membranes influence growth, shape and differentiation of cells and tissues. However, the role and influence of Descemet's membrane during corneal development is not understood. To address this question, the relationships between cell growth and fibronectin, laminin and actin distribution in the developing rat corneal endothelium in vivo has been examined. During fetal development, rat ...

OBJECTIVE To establish a reproducible method for the culture of primary equine corneal epithelial cells, keratocytes, and endothelial cells and to describe each cell's morphologic characteristics, immunocytochemical staining properties and conditions required for cryopreservation. PROCEDURES Corneas from eight horses recently euthanized for reasons unrelated to this study were collected asept...

PURPOSE To report a case of corneal decompensation due to the Ex-PRESS(®) mini glaucoma shunt device (Ex-PRESS). PATIENT AND METHODS A 75-year-old man had pseudoexfoliation glaucoma in his right eye. He underwent filtration surgery with Ex-PRESS. His intraocular pressure was 7 mmHg after 9 months. RESULTS We observed partial decompensation of the corneal endothelium adjacent to the filterin...

In tissue bank, one of the key steps in corneal grafts sorting is analysis deepest cell layer cornea, endothelium. This consists counting density living cells using trypan blue staining. However, this dye toxic and measurement localized. Then no information given on cell’s viability entire layer. preliminary study proposes to track kinetics variation scattered light during deturgescence process...

PURPOSE To assess corneal structure and the effects of acute hyperglycemia on corneal function in subjects with type 1 diabetes. METHODS Twenty-one diabetic and 21 nondiabetic volunteers of similar age were recruited. Baseline measurements of intraocular pressure (IOP), corneal thickness (CT), corneal autofluorescence (CAF), corneal sensitivity (CST), central and temporal endothelial cell den...

OBJECTIVE To describe the corneal endothelial density and morphology in patients of Phramongkutklao Hospital and the relationship between endothelial cell parameters and other factors. METHODS Four hundred and four eyes of 202 volunteers were included. Noncontact specular microscopy was performed after taking a history and testing the visual acuity, intraocular pressure measurement, Schirmer'...

AIMS To compare two different techniques for preparation of pre-cut lamellar corneal tissue for Descemet stripping automated keratoplasty. BACKGROUND/METHODS Eight donor lenticules were created with a full pass of the microkeratome blade, which resulted in a posterior lamella and a free cap (anterior lamella off group (AL-off)). Contralateral donor lenticules were created with an incomplete p...