PURPOSE To compare the endothelial structure and thickness of the cornea in diabetic and nondiabetic patients, and to evaluate the systemic and ocular factors that contribute to the damage of endothelial cells in diabetic patients. METHODS The corneal endothelial structure and central corneal thickness (CCT) were investigated in 99 type II diabetic patients (99 eyes) and in 97 nondiabetic pat...

PURPOSE To determine the efficacy of the angiogenic inhibitor TNP-470 on inflammatory corneal neovascularization. Topical and systemic delivery of the drug were investigated in a murine model as well as inhibition of endothelial cell proliferation in vitro and in vivo. METHODS The effect of TNP-470 on VEGF- and bFGF-stimulated bovine capillary endothelial (BCE) cell proliferation was evaluate...

OBJECTIVE To evaluate the effects of phacoemulsification energy on the redox state and mitochondrial distribution of cultured human endothelial cells. METHODS Human corneal endothelial cells from fresh banked human donor tissue not suitable for transplantation were harvested and cultured. Cellular autofluorescence images were obtained using an inverted microscope. The redox fluorometric ratio...

BACKGROUND/AIMS To compare two intraocular irrigating solutions, Balanced Salt Solution Plus (BSS Plus) versus Lactated Ringer's (Ringer), for the preservation of corneal integrity after phacoemulsification. METHODS 110 patients undergoing phacoemulsification were randomised to either BSS Plus (n=55) or Ringer (n=55) as the irrigating solution. Patients were examined at baseline and at 1, 8, ...

purpose: to assess the efficacy and safety of laser in situ keratomileusis (lasik) for correction of residual myopia and astigmatism after penetrating keratoplasty (pk). methods: in this prospective interventional case series, lasik was performed on 14 eyes of 13 patients unable to wear glasses or contact lenses after pk. the interval between lasik and pk was at least one year. all patients had...

purpose: to evaluate the effect of human amniotic fluid (haf) on the growth of human corneal endothelial cells (hcecs) and to establish an in vitro method for the expansion of hcecs. methods: hcecs were cultured in dmem-f12 supplemented with 20% fetal bovine serum (fbs). confluent monolayer cultures were trypsinized and passaged using 20% fbs- or 10%, 20%, or 30% haf-containing media. cell prol...

Corneal endothelial dysfunction is a major cause of visual impairment, since these cells maintain corneal transparency. Although surgical techniques have been developed to replace abnormal corneal endothelium, these procedures are technically difficult and challenging due to a shortage of donor corneas. Regenerative medicine uses specially grown tissues to replace injured ones and has been succ...

■ Fuchs endothelial dystrophy (FED) is a progressive disorder of the corneal endothelium with accumulation of focal excrescences called guttae and thickening of Descemet’s membrane, leading to stromal edema and loss of vision ■ The inheritance of FED is autosomal dominant, with modifiers such as increased prevalence in the elderly and in females ■ Corneal endothelial cells are the major “pump” ...

purpose : we describe our experience on safety and effectiveness of phacoemulsification in cataract and congenital iris coloboma and point out some specific surgical recommendations aimed to minimize its complications. methods : a prospective case series study was conducted on nineteen consecutive patients with cataract and congenital iris coloboma referred to the farabi eye hospital in tehran....

BACKGROUND Wound healing of the endothelium occurs through cell enlargement and migration. However, the peripheral corneal endothelium may act as a cell resource for the recovery of corneal endothelium in endothelial injury. AIM To investigate the recovery process of corneal endothelial cells (CECs) from corneal endothelial injury. METHODS Three patients with unilateral chemical eye injurie...