Saffron (Crocus sativus L.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of Cellulase, Pectinase and Hemicellulase and embedded in Ca-alginate beads. They were cultured with or without nurse cells in MS medium supplemented with 2,4-D and 6-benzylaminopurine at 25°C. After several changes of medium, cell-clusters appeared on the surface of the Ca-algi...

The purpose of this paper is to analyze the simultaneous effect of Corporate Culture and market orientation on the performance of small to medium-sized industrial manufacturing firms. To this end, a cross-sectional survey and variance based structural equation modeling was used for testing the hypotheses. The samples were selected based on a stratified sampling of commodity and specialty indust...

To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...

Introduction Pre-clinical testing of hemiarthroplasty devices requires that the tribological conditions present in vivo with live cartilage be closely duplicated. A current limitation in the tribological testing of live cartilage involves the use of cell-culture media as lubricant. Study Aim to develop and test a new hyaluronan-phospholipid based medium (HA-phospholipid medium) that combines ...

Translucent culture media are essential for the determination of gonococcal colony types. In this study two hemoglobin-free, translucent culture media were compared with Thayer-Martin (TM) medium for the primary isolation of Neisseria gonorrhoeae from male urethral specimens. The antibiotic-free translucent medium (GCB) and the translucent medium containing vancomycin, colistin, and nystatin (V...