Fast and reliable assays to precisely define the nature of the infectious agents causing sepsis are eagerly anticipated. New molecular biology techniques are now available to define the presence of bacterial or fungal DNA within the bloodstream of sepsis patients. We have used a new technique (VYOO®) that allows the enrichment of microbial DNA before a multiplex polymerase chain reaction (PCR) ...

ABSTRACT
 Objectives
 To compare mean platelet volume (MPV) in neonates with and without sepsis. 
 Study design
 Cross sectional descriptive study.
 Place & duration of study
 Neonatal Unit, Department Paediatrics, Military Hospital, Rawalpindi from 2nd July 2017 to 22nd January 2018.
 Material Methods
 140 neonates, either gender ages between 0-28 da...

neonatal sepsis, a life-threatening condition, presents with non-specific clinical manifestations and needs immediate empirical antimicrobial therapy. choosing an appropriate antibiotic regimen covering the most probable pathogens is an important issue. in this study we compared the effectiveness of ceftizoxime and amikacin in the treatment of neonatal sepsis both in combination with ampicillin...

OBJECTIVE The objective of this study was to evaluate the relationship between histological chorioamnionitis and laboratory markers of infection and congenital sepsis in very-low-birth-weight (VLBW) premature neonates. METHOD This study is a retrospective review of laboratory results of VLBW neonates with birth weight less than 1500 g in our neonatal intensive care unit (NICU) in the last 5 y...

OBJECTIVE To compare the use of a generic molecular assay to 'standard' investigations used to assist the diagnosis of late onset bacterial sepsis in very low birth weight infants (VLBW, <1500 g). METHODS VLBW infants, greater than 48 hours of age, who were clinically suspected to have sepsis were investigated using standard tests (full blood count, C-reactive protein (at presentation) and bl...

Background Globally, 4 million neonates die annually, with one-third of such deaths occurring as a result of infections. In 2011, there were 7.2million deaths in children below 5 years globally, and a proportion of 40% of these deaths occurred in neonates. Sepsis was reported to account for one-third of these deaths. Presently, multidrug antibiotic resistance is rapidly increasing in Neonatal I...

The increasing incidence of Vancomycin resistant Enterococcci (VRE) infection is of concern because of limited treatment options and increased mortality. Here we report a rare case of Vancomycin resistant Enterococccal neonatal sepsis. A Very low birth weight male neonate with respiratory distress and suspected hyaline membrane disease was admitted to NICU had pulmonary haemorrhagia and suspect...

BACKGROUND Neonatal septicaemia diagnosis based on clinical features alone is non-specific leading to the initiation of unnecessary antibiotic treatment posing a danger of increased antibiotic resistance. In the present study the utility of serial qualitative C-reactive protein (CRP) assay and white blood cells count (WBC) in the diagnosis of neonatal septicaemia was investigated using blood cu...

Rapid identification of infection has a major impact on the clinical course, management, and outcome of critically ill intensive care unit (ICU) patients. We compared the results of PCR and procalcitonin with blood culture for ICU patients suspected of having septicemia. Ninety patients (60 patients meeting the criteria for sepsis and 30 patients not meeting the criteria for sepsis) were evalua...

OBJECTIVE To determine the validity of C-reactive protein levels for diagnosis of neonatal sepsis. METHODS A cross sectional (Validation) study was conducted at Neonatology unit in KRL general hospital (emergency/OPD) of 7 months duration from February 2012 to August 2012. By using purposive sampling technique, 147, sample size was calculated by using WHO sample size calculator taking sensiti...