نتایج جستجو برای: culture techniques
تعداد نتایج: 866537 فیلتر نتایج به سال:
AIMS To improve detection of neuroblastoma metastases in the bone marrow: morphological evaluation of bone marrow is a routine and important component of the clinical staging of neuroblastoma and it depends on the successful identification of tumour cells which may only be present at extremely low levels. METHODS Bone marrow mononuclear cells from patients with neuroblastoma were incubated in...
Since the discovery by Enders, Weller, and Robbins in 1949' that poliovirus could multiply in cultures of non-nervous tissues, cell culture techniques have been used extensively in the studies of enteroviruses' for the last decade. The accumulated literature reveals that the usefulness of these culture methods is many-fold and may include virus isolation, virus multiplication studies, and serol...
The identification of fluorescently stained cell nuclei is the basis of cell detection, segmentation, and feature extraction in high content microscopy experiments. The nuclear morphology of single cells is also one of the essential indicators of phenotypic variation. However, the cells used in experiments can lose their contact inhibition, and can therefore pile up on top of each other, making...
We present a highly parallel microfluidic approach for contacting single cell pairs. The approach combines a differential fluidic resistance trapping method with a novel cellular valving principle for homotypic and heterotypic single cell co-culturing. Differential fluidic resistance was used for sequential single cell arraying, with the adhesion and flattening of viable cells within the micros...
Polyphenols are a diverse group of micronutrients from plant origin that may serve as antioxidants and that contribute to human health in general. More specifically, many research groups have investigated their protective effect against cardiovascular diseases in several animal studies and human trials. Yet, because of the excessive processing of the polyphenol structure by human cells and the ...
Central neurons develop and maintain molecularly distinct synaptic specializations for excitatory and inhibitory transmitters, often only microns apart on their dendritic arbor. Progress towards understanding the molecular basis of synaptogenesis has come from several recent studies using a coculture system of non-neuronal cells expressing molecules that generate presynaptic or postsynaptic "he...
The conventional virus isolation technique was compared with a 24-h shell vial centrifugation culture technique and with a 48-h tube culture method for the detection of cytomegalovirus (CMV) in MRC-5 cells. Of 200 clinical specimens tested, 41 were positive for CMV by at least one procedure. Indirect immunoperoxidase staining was positive for 32 (78.0%) of 41 specimens in the tube culture metho...
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