To facilitate the study of individual metal binding sites of polymeric metalloproteins, conversion of exchange-labile Co(II) in E. coli alkaline phosphatase (EC 3.1.3.1) to exchange-inert Co(III) was examined. Oxidation of Co(II) alkaline phosphatase with hydrogen peroxide results in a single absorption maximum at 530 nm and loss both of the characteristic electron paramagnetic signal and of en...

The supramolecular approach is becoming increasingly dominant in biomimetics and chemical catalysis due to the expansion of enzyme active center idea, which now includes binding cavities (hydrophobic pockets), channels canals for transporting substrates products. For a long time, mimetic strategy was mainly focused on first coordination sphere metal ion. Understanding that highly organized cavi...

In this paper, ‘‘metallomics’’ is proposed as a new scientific field in order to integrate the research fields related to biometals. Metallomics should be a scientific field in symbiosis with genomics and proteomics, because syntheses and metabolic functions of genes (DNA and RNA) and proteins cannot be performed without the aid of various metal ions and metalloenzymes. In metallomics, metallop...

Mesothelial cells play a critical role in the remodeling process that follows serosal injury. Although mesothelial cells are known to synthesize a variety of extracellular matrix components including types I, III, and IV collagens, their potential to participate in matrix degradation has not been explored. We now report that human pleural and peritoneal mesothelial cells express interstitial co...