نتایج جستجو برای: cyt c

تعداد نتایج: 1057486  

Journal: :Biochemistry 2003
Osamu Miyashita José N Onuchic Melvin Y Okamura

Electrostatic interactions are important for protein-protein association. In this study, we examined the electrostatic interactions between two proteins, cytochrome c(2) (cyt c(2)) and the reaction center (RC) from the photosynthetic bacterium Rhodobacter sphaeroides, that function in intermolecular electron transfer in photosynthesis. Electrostatic contributions to the binding energy for the c...

2017
CM Figueroa BN Suárez AM Molina JC Fernández Z Torres K Griebenow

Herein we tested a nanosized cancer-cell targeted delivery system based on cytochrome c (Cyt c) and hyaluronic acid. Cyt c was chosen since it is a per se non-toxic protein but causes apoptosis when delivered to the cytoplasm of target cells. Hyaluronic acid was employed to create the nanosized delivery system with passive targeting capability in order to exploit the enhanced permeation and ret...

Journal: :ARQ (Santiago) 2018

Journal: :Frontiers in veterinary science 2015
Leila Hadj-Henni Thibaut De Meulemeester Jérôme Depaquit Philippe Noël Adeline Germain Remi Helder Denis Augot

To date, studies on host preferences and blood meal identification have been conducted for Culicoides species using molecular-based methods such as PCR techniques to amplify only a fragment from universal vertebrate mitochondrial genes such as cytochrome c oxidase subunit I or cytochrome b (Cyt b). The vertebrate prepronociceptin gene (PNOC) was also tested in this field. However, the choice of...

Journal: :Molecular and cellular biology 2003
Hila Toledano-Katchalski Zohar Tiran Tal Sines Gidi Shani Shira Granot-Attas Jeroen den Hertog Ari Elson

cyt-PTP epsilon is a naturally occurring nonreceptor form of the receptor-type protein tyrosine phosphatase (PTP) epsilon. As such, cyt-PTP epsilon enables analysis of phosphatase regulation in the absence of extracellular domains, which participate in dimerization and inactivation of the receptor-type phosphatases receptor-type protein tyrosine phosphatase alpha (RPTPalpha) and CD45. Using imm...

Journal: :Bio-medical materials and engineering 2015
Tian Yang Yu-Shuai Zhang Xiao-Lu Yang Fang-Yong Geng Bao-Lin Xiao Meng-Meng Xu Di Li Jun Hong Ali Akbar Moosavi-Movahedi

A novel artificial peroxidase (AP) with highly catalytic efficiency was designed using hexadecyl trimethyl ammonium bromide (CTAB, 3 mM) nano-micelles and bovine heart cytochrome c (Cyt c, 0.5 µM) in 100 mM, pH 8.0 phosphate buffer at 25°C. The catalytic rate (kcat) and Michaelis-Menten (Km) of the AP were determined to be 0.311 ± 0.013 s(-1) and 8.64 ± 0.6 µM. The catalytic efficiency was 0.03...

Journal: :Biophysical journal 2011
Y Min T F Alig D W Lee J M Boggs J N Israelachvili J A Zasadzinski

Monolayers based on the composition of the cytoplasmic (CYT) or extracellular (EXT) sides of the myelin bilayer form coexisting immiscible liquid phases similar to the liquid-ordered/liquid-disordered phases in phospholipid/cholesterol monolayers. Increasing the temperature or surface pressure causes the two liquid phases to mix, although in significantly different fashion for the CYT and EXT m...

2011
Wenxian Lan Zhonghua Wang Zhongzheng Yang Jing Zhu Tianlei Ying Xianwang Jiang Xu Zhang Houming Wu Maili Liu Xiangshi Tan Chunyang Cao Zhong-Xian Huang

To convert cyt c into a peroxidase-like metalloenzyme, the P71H mutant was designed to introduce a distal histidine. Unexpectedly, its peroxidase activity was found even lower than that of the native, and that the axial ligation of heme iron was changed to His71/His18 in the oxidized state, while to Met80/His18 in the reduced state, characterized by UV-visible, circular dichroism, and resonance...

2014
Mini Kamboj N. Esther Babady Jane W. Marsh Jessica L. Schlackman Crystal Son Janet Sun Janet Eagan Yi-Wei Tang Kent Sepkowitz

BACKGROUND The use of molecular methods to diagnose Clostridium difficile infection (CDI) has improved diagnostic yield compared to conventional methods. However, PCR testing can detect colonization and has introduced several practical challenges pertaining to need for treatment and isolation of cases. METHODS For all new cases detected by real-time PCR, concurrent cytotoxin assay was perform...

Journal: :Advanced Functional Materials 2023

Apoptosis is the natural programmed cell death process, which responsible for abnormal clearance. However, many cancer cells develop various mechanisms to escape apoptosis through interrupting apoptosome assembly, a key step initiate apoptosis. This promotes tumorigenesis and drug resistance, thus, poses great challenge in treatment. Herein, biomimetic lipid nanocarrier mimicking mitochondrial ...

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