The qcr of Bacillus stearothermophilus K1041 encoding three subunits of the quinol-cytochrome c oxidoreductase (cytochrome reductase, b6c1 complex) was cloned and sequenced. The gene (qcrA) for a Rieske FeS protein of 19,144 Da with 169 amino acid residues, and the gene (qcrC) for cytochrome c1 of 27,342 Da with 250 amino acid residues were found at adjacent upstream and downstream sides of the...

The chlorophyll-containing lamellar structures isolated from Anabaena variabilis have the ability to oxidize reduced mammalian cytochrome c in the dark. This activity is oxygen dependent and heat labile. As with other cytochrome oxidase preparations, activity is stimulated by detergents and is sensitive to the ionic strength of the assay solution. The cytochrome oxidase activity is not inhibite...

Cytochrome P-450 was purified to a specific content of 16 nmol/mg of protein from bovine adrenal cortex microsomes by hydrophobic chromatography using an w-amino-n-octyl Sepharose column in the presence of Emulgen 913, a nonionic detergent. The purified preparation exhibited a single polypeptide band (Mr = 47,000 f 1,000) when submitted to sodium dodecyl sulfate-polyacrylamide gel electrophor...

AIMS To evaluate the mitochondrial respiratory enzyme activities in blood cells of Leber's hereditary optic neuropathy (LHON) with 11778 point mutation of mitochondrial DNA. METHODS Assays for the activities of NADH-cytochrome c reductase (complex I+complex III), succinate-cytochrome c reductase (complex II+complex III), and cytochrome c oxidase (complex IV) on blood cell mitochondria of seve...

Irradiation of cells of Prototheca zopfii with blue light inhibited the respiratory capacity of the cells. The inhibition of respiration was correlated with a photodestruction of cytochrome c(551), cytochrome b(559), and cytochrome a(3). Cytochrome c(549), cytochrome b(555), and cytochrome b(564) were unaffected by the irradiation treatment. The alpha-band of reduced cytochrome a was shifted fr...

Neutrophil plasma membranes from patients with the X-linked and autosomal recessive forms of chronic granulomatous disease (CGD) that lack cytochrome b are incapable of generating superoxide anion (02-) in vivo and in vitro. The 02generating activity of these defective membranes was reconstituted with the addition of partially purified human neutrophil cytochrome b in a detergent-based, cell-fr...

Sodium deoxycholate and sodium cholate have been widely used to solubiliie mitochondrial enzymes ever since their use by Keilin and Hartree for clarifying heart muscle preparations (l), by Hopkins, Lutwak-Mann, and Morgan (2) for extracting succinic dehydrogenase, by von Euler and Hellstrom (3) for preparing cytochrome b and cytochrome oxidase (cytochrome c:Os oxidoreductase, EC 1.9.3.1), and b...