A commercial multiplex real-time PCR assay (Cepheid Xpert C. difficile assay) for the diagnosis of Clostridium difficile infection was evaluated. The sensitivity and specificity of the Cepheid assay were 97.1% and 93.0% for fresh stools, using the cell cytotoxicity neutralization assay as the reference. Using PCR ribotyping as the reference for ribotype 027 strains, the corresponding figures we...

To identify potential antivirals against BTV, we have developed, optimized and validated three assays presented here. The CPE-based assay was the first assay developed to evaluate whether a compound showed any antiviral efficacy and have been used to screen large compound library. Meanwhile, cytotoxicity of antivirals could also be evaluated using the CPE-based assay. The dose-response assay wa...

Abstract Background Myocardial injury is the main manifestation of cardiovascular diseases, and previous studies have shown that propofol (PPF) regulates myocardial injury. However, mechanism PPF in regulating remains to be further explored. This work aims analyze effects on human cardiomyocyte underlying mechanism. Methods The regulatory functional role circAPBB2 were analyzed using an vitro h...

Cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. Therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-CdA) on the breast cancer cell line, MCF-7 (estrogen receptor positive, ER+). MTT assay, annexin V-Fluorescein/PI and Hoechst 33...

In order to observe the cytotoxicity of Acanthamoeba spp., which were isolated from contact lens containers as ethiological agents for the probable amoebic keratitis in Korea, the crystal violet staining method and LDH release assay were carried out. In the crystal violet staining method, among eight contact lens container isolates, isolate 3 (Acanthamoeba KA/LS5) showed 83.6% and 81.8% of cyto...

background: a strong antimicrobial synergism between chlorhexidine (chx) and hydrogen peroxide (h2o2) has been reported, but there is not enough data on the cytotoxicity of this combination. the primary aim of this study was to evaluate the cytotoxicity of chx-h2o2 combination in different concentrations and secondary aim is to assess the infl uence of h2o2 on cytotoxicity of chx on cultured hu...

The major challenge to treat Parkinson’s disease (PD) is penetration of target molecule into the brain to improve the efficacy of drugs. To achieve better brain penetration and targeted delivery, 1,9-Pyrazoloanthrone (1,9-P) loaded liposomes were developed by solvent injection technique using ultrasonication and evaluated for particle size, morphology, entrapment efficiency, FT-IR, and in-vitro...

background: cadmium is one of the most toxic heavy metals in our environment having a very strong ability to accumulate in body organs, especially in kidney. the present study was done to determine the genotoxicity and cytotoxicity in kidneys of rats exposed to cadmium. methods: male rats (n=30), kept in standard conditions were used in this study. the animals were randomly divided into 2 group...

Objective: To evaluate the cytotoxicity and antibacterial effect of bleached turmeric hydro-alcoholic extractcompared to sodium hypochlorite as an endodontic irrigant. Material Methods: Cytotoxicity was evaluatedon human skin fibroblasts using MTT assay. The irrigants were tested at 1, 5- 15-minutes time intervals. Aftercontact time, solution added well plates incubated. After incubation period...

introduction: the aim of this study was to compare the cytotoxicity of proroot mta (pmta), root mta (rmta) and portland cement (pc) on human gingival fibroblasts (hgfs). materials and methods: the extracts of the test materials were placed close to human gingival fibroblasts (hgfs) within 96-well plates. cellular viability was assessed using mtt assay in different intervals (freshly mixed, 4, 2...