Thrombosis and/or disseminated intravascular coagulation (DIC) are complications specifically associated with the use of factor IX complex in some patients. Assuming that these complications might result from zymogen overload, we have produced, using diethylaminoethyl (DEAE)-Sephadex (Pharmacia, Piscataway, NJ) and sulfated dextran chromatography, a factor IX concentrate (coagulation factor IX)...

Three established cell lines were examined for growth on a newly developed microcarrier which consists of glass beads. The cells were simultaneously examined for growth on comrnercially available microcarriers made from DEAE-dextran and from plastic. Cell yields on the glass microcarriers were comparable to the cell yields on the commercially available products. Cells grown on the glass microca...

A Ca2+-activatable cyclic nucleotide phosphodiesterase from bovine heart can be eluted from a DEAE-cellulose column either in the free form by buffers containing 0.1 mM ethylene glycol bis(beta-aminoethyl ether)N-N,N'N'-tetraacetic acid (EGTA) or as a complex of the enzyme with its protein modulator by buffers containing 0.01 mM CaCl2. A purification procedure based primarily on the significant...

Cholestyramine is the most widely used bile acid sequestrant in the treatment of hypercholesterolemia. However, cholestyramine has unpleasant side effects as a consequence of its hydrophobic backbone. Therefore, high-capacity bile acid sequestering biopolymers with cationic chitosan derivatives were developed, because electrostatic interactions are important for binding with bile acid anions. D...

It has recently been recognised that vaccine adjuvants play a critical role in directing the nature of a vaccine induced effector response. In the present study, several adjuvants were evaluated for their ability to protect sheep after field vaccination with the larval-specific Haemonchus contortus antigen, HcsL3. Using a suboptimal antigen dose, aluminium adjuvant was shown to reduce the cumul...

Salim Mattar, Camilo Guzmán, Justiniano Arrazola, Ella Soto, José Barrios, Noemí Pini, Silvana Levis, Jorge Salazar-Bravo, and James N. Mills Author affi liations: Universidad de Córdoba, Montería, Colombia (S. Mattar, C. Guzmán, J. Arrazola, E. Soto, J. Barrios); Instituto Nacional de Enfermedades Virales Humanas “Dr. Julio I Maiztegui,” Pergamino, Argentina (N. Pini, S. Levis); Texas Tech Uni...

Author affi liations: Etablissement Français du Sang Alpes-Méditerranée, Marseille, France (P. Biagini, M. Touinssi, C. Picard, P. Gallian, J.-F. Cantaloube, P. de Micco); Université de la Méditerranée, Marseille (P. Biagini, M. Touinssi, P. Gallian, J.-F. Cantaloube, P. de Micco); Centre Hospitalier Universitaire, Marseille (B. Dussol); Hôpital de la Conception, Assistance Publique de Marseill...