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25 In the physiological condition, mammalian oocytes and embryos appears to be stimulated not only chemically but also mechanically, such as compression, shear stress and/or friction force in the follicle and female reproductive tract. The present study was undertaken to examine the effects of kinetic culture with a tilting device in chemically defined media during in vitro maturation (IVM) of ...

Dendritic cells produce IL-12 both in response to microbial stimuli and to T cells, and can thus skew T cell reactivity toward a Th1 pattern. We investigated the capacity of dendritic cells to elaborate IL-12 with special regard to their state of maturation, different maturation stimuli, and its regulation by Th1/Th2-influencing cytokines. Monocyte-derived dendritic cells were generated with GM...

BACKGROUND Crocin is an active ingredient of saffron (Crocus sativus L.) and its antioxidant properties have been previously investigated. This carotenoid scavenges free radicals and stimulates glutathione (GSH) synthesis; consequently, it may protect cells against oxidative stress. The aim of this research is to protect oocytes from oxidative stress by the addition of a natural source antioxid...

Details in the fermentation of oxytetracycline in a synthetic medium with Streptomyces rimosus have been presented. In these studies, an organic nitrogen source was shown to be essential for the production of significant amounts of antibiotic activity. Of the amino acids tested, aspartic acid, proline, threonine, valine, and beta-alanine were utilized well for both growth and antibiotic product...

result from other techniques, such as those requiring aeration of cultures. The exhaust air from such operationls must be sterilized and the most reliable means of doing this is incineration. The 1 cfm capacity of the incinerator described above is sufficient for mnost laboratory scale operations. If provision is made for cooling the exit air, the apparatus may be employed as a source of steril...

New media have made available a wide range of platforms and content choices. However, audiences cope with abundant choices by using more narrowly defined repertoires. Unfortunately, we know little of how users create repertoires across media platforms. This study uses factor analysis to identify user-defined repertoires from data obtained by following 495 users throughout an entire day. Results...

Cultivation of amebae of the axenic strain of Naegleria gruberi, NEG-M, was achieved in media consisting entirely of chemically defined components. A complete medium that contains 31 components allows growth with yields up to 5 x 10(6) amebae per ml. A minimal medium gives lower yields but defines 22 components that are essential for continuous cultivation: 11 amino acids, 6 vitamins, hematin, ...

The selection of a chemically defined medium for nutritional or physiological studies depends upon the objectives of the investigator. The four chemically defined media for the cultivation of brucellae that have been reported during the past decade have tended toward one or the other of two objectives. Either multiple amino acid nitrogen source media have been devised and used to establish the ...