Post-traumatic stress disorder (PTSD) has been associated with dysregulation of the neuroendocrine system. In this study we examine the effects of psychotherapy in 21 PTSD patients, with and without coexisting depression, on the levels of six stress-related hormones: cortisol, dehydroepiandrosterone (DHEA), and dehydroepiandrosterone-sulfate (DHEA-S), prolactin, thyrotropin (TSH) and free thyro...

D (DHEA) was isolated in urine in 1934, and DHEA 3b-sulfate (DHEAS) was identified 10 years later (1, 2). It took another decade to identify DHEA and DHEAS in peripheral blood (3, 4). More recently, fatty acid esters of DHEA, testosterone, and estradiol (5) were isolated from a variety of tissues, particularly fat (6), and their presence in circulation (5). In 1981, Corpéchot et al. (7) reporte...

Although heated strong acid hydrolysis has been generally adopted as the routine procedure for the hydrolysis of urinary steroids, it has disadvantages such as the destruction of the steroids, the production of artifacts, and the remarkable changes of the pattern of the fractionation. As for the glucuronide types of the urinary steroids, the .-glucuronidase method can now be used instead of thi...

The characterization and quantification of pregnenolone in human sciatic nerves were undertaken, following previous demonstration of the synthesis of this steroid in rat brain oligodendrocytes, to explore the hypothesis that Schwann cells may demonstrate the same biosynthetic activity. Pregnenolone was definitively identified by mass spectrometry and quantified by specific radioimmunoassay. Its...

Abstract The pathophysiology of COVID comprises an exaggerated pro-inflammatory response. Hypothalamic-pituitary-adrenal (HPA) axis has a crucial role in various inflammatory conditions and modulated immunological Limited evidence is available regarding the incidence effect HPA dysfunction COVID-19. Although cortisol levels have only been estimated few studies, dehydroepiandrosterone sulfate (D...

The laboratory reliability and validity of sex hormone measurements were examined at multiple levels, including lower levels characteristic of children and postmenopausal women. Serum was drawn from four adult male and four adult female healthy volunteers. From each individual's serum pool, a medium- and a low-dilution pool were created. Biochemical analyses for total and non-sex hormone-bindin...

We have developed a rapid and cost-effective enzyme immunoassay for dehydroepiandrosterone sulfate (DHEA-S) in plasma, performed with samples on a microtitre plate within 2.5 h. No extraction or centrifugation steps are involved. The 3-hemisuccinate of dehydroepiandrosterone is labeled with horseradish peroxidase, then mixed with hydrogen peroxide substrate in the presence of the chromogen, tet...