the influence of long-term mixed organic and inorganic contamination on soil microbial activity, community structure and genetic diversity was investigated in soil samples from a coke oven plant located in upper silesia, poland. the tested soils were heavily contaminated with polycyclic aromatic hydrocarbons (pahs) and heavy metals. the microbial communities were characterized using the phospho...

Source and Description of Primers. Primers GHRE102′Fgc and GHRE102′R were designed based on the cDNA sequence of the bovine growth hormone receptor (GHR) gene (Hauser et al., 1990) and the genomic DNA sequence of the human GHR gene (Godowski et al., 1989). Primers GHRE10F and GHRE10R were designed based on our own sequence of the fragment amplified using the first pair of primers. Primer Sequen...

The aim of this study was to determine the activity and structure microbial communities in soils contaminated with heavy metals (HMs). To achieve goal, soil samples were taken from two sites (i.e., Piekary Śląskie Bukowno) Poland. A wide range methods applied, including: total metal-tolerant culturable bacteria enumeration; community analysis using phospholipid fatty acid method (PLFA); denatur...

Methods: Exons of the two genes were screened for mutations by denaturing gradient gel electrophoresis (DGGE) and/or single strand conformation polymorphism electrophoresis (SSCP); any variants were sequenced directly. Results :Although many sequence variants were found in both genes, none could be associated with disease. Additionally, the gene structure and sequence of the coding exons of GNB...

Denaturing gradient gel electrophoresis (DGGE) was used to probe for mutations associated with pyrazinamide (PZA) resistance in the pncA gene of Mycobacterium tuberculosis. DGGE scans for mutations across large regions of DNA and rivals sequencing in its ability to detect DNA alterations. Specific mutations can often be recognized by their characteristic denaturation pattern, which serves as a ...

Bacteria play an important role in the initiation and progression of periodontal diseases and are part of a biofilm, which can contain over 100 different species. The aim of the present study was to show the potential of denaturing gradient gel electrophoresis (DGGE) as a tool for the detection of clinically relevant species and to compare the results of detection by DGGE with those by PCR and ...

In two-dimensional denaturing gradient gel electrophoresis, DNA is digested with a restriction endonuclease and the resulting DNA fragments are separated as a function of size by conventional agarose gel electrophoresis. Following this first dimension electrophoresis, the fragment distribution is placed at the top of a denaturing gradient slab gel and electrophoresis is carried out parallel to ...

Relationships between community composition of the iron-reducing Geobacteraceae, pollution levels, and the occurrence of biodegradation were established for an iron-reducing aquifer polluted with landfill leachate by using cultivation-independent Geobacteraceae 16S rRNA gene-targeting techniques. Numerical analysis of denaturing gradient gel electrophoresis (DGGE) profiles and sequencing reveal...