The carboxylation of pyruvate in the absence of acetylCoA by sheep kidney pyruvate carboxylase has been demonstrated and the characteristics of the reaction have been investigated. Under optimal conditions, the maximal specific activity for the reaction was 25 % of that obtained in the presence of saturating concentrations of acetyl-CoA. The reaction was markedly stimulated by K+, NH4+, Rb+, an...

For rhizobacteria to exert physiological effects on plant growth, the bacteria must first effectively colonize the root surface. To examine the relationship between long-term colonization of root systems and adherence to roots in the short term, a binding assay was developed. Adherence was determined by incubating roots of intact radish seedlings with bacteria, washing and homogenizing the root...

The complexation of Alkali metal cations by the water-soluble p-sulfonic acid calix(4)arenewas thermodynamically characterized using spectrophotometeric data which are consistentwith the formation of a 1:1 complex resulting from electrostatic interactions between thesulfonato groups and alkali metal cations. In this study, we determined the formationconstants (log K) of the complexes and have c...

We have extended our investigations of the multiple binding modes that form between the Escherichia coli single strand binding (SSB) protein and single-stranded DNA (Lohman, T. M. & Overman, L. B. (1985) J. Biol. Chem. 260, 3594-3603; Bujalowski, W. & Lohman, T. M. (1986) Biochemistry 25, 7799-7802) by examining the effects of anions, pH, BaCl2, and protein binding density on the transitions am...

the complexation of alkali metal cations by the water-soluble p-sulfonic acid calix(4)arenewas thermodynamically characterized using spectrophotometeric data which are consistentwith the formation of a 1:1 complex resulting from electrostatic interactions between thesulfonato groups and alkali metal cations. in this study, we determined the formationconstants (log k) of the complexes and have c...

cGMP-dependent protein kinase from bovine lung has been purified to homogeneity using 8-(2-aminoethyl)-amino adenosine 3':5'-monophosphate/Sepharose. Conditions for adsorption of holoenzyme to the affinity chromatography media followed by competitive ligand elution with cGMP have been determined. The holoenzyme of 150,000 molecular weight is composed of two 74,000 molecular weight subunits whic...