Pancreatic lipase requires colipase, a protein cofactor, to counteract the in vitro inhibition by bile salt. Lipase activity is inhibited by nonsteroidic detergents regardless of their charge and structure. Detergent-inhibited lipase is reactivated by colipase but in all cases activation is limited to a narrow range of detergent concentration. Complementary studies on the bile salt and detergen...

A pooled sample of hair was divided and portions prepared for analysis by three washing procedures, to evaluate the effect of washing procedure on the subsequent trace-element (Zn, Cu, Mg) content. The methods selected were a detergent wash, a hexane-ethanol wash, and an acetone-ether-detergent wash. For all elements, there was a significant difference among the results after these wash procedu...

The Smiths are busy raising two preschool children and managing multiple responsibilities at home and at work. They juggle household chores and child care on evenings and weekends. Like most Canadians, they strive to provide a safe home environment for their children. Laundry products are stored on a high shelf with other household chemicals. One evening, while preparing to do the laundry, Mr S...

The most important physico-chemical characteristics describing detergent micelles are the CMC value and the aggregation number. These parameters depend critically on external conditions such as pH, ionic strength, buffer composition or temperature. However, the influence of these conditions on CMC or aggregation number is a priori not to predict, but the most important parameter. This holds esp...

Lipases from Candida rugosa (CRL) and lipase isoforms A and B from Candida antarctica (CAL-A and CAL-B) were adsorbed on aminated supports in the presence of detergents to have individual lipase molecules. Then, one fraction was washed to eliminate the detergent, and both preparations were treated with glutaraldehyde. The presence of detergent during the cross-linking of the lipases to the supp...

PRINCIPAL FINDINGS HEK293 cells stably expressing PTX-insensitive δ-opioid receptor-Gi1α (C351I) fusion protein were homogenized, treated with low concentrations of non-ionic detergent Brij-58 at 0°C and fractionated by flotation in sucrose density gradient. In optimum range of detergent concentrations (0.025-0.05% w/v), Brij-58-treated, low-density membranes exhibited 2-3-fold higher efficacy ...

The aim of this experiment is to investigate the effects lactic acid bacteria and cellulase on fermentation quality, chemical composition, aerobic stability ruminal degradation characteristics mixed silage prepared with amaranth rice straw. Lactic were used as additives, four treatments follows: control group (CON, no additive), (LAB, additive amount was 5 mg/kg fresh matter), (CEL, 2 mg/kg) (L...