Previous studies have shown that substitution of single amino acid residues in human Melan-A immunodominant peptides Melan-A27-35 and Melan-A26-35 greatly improved their binding and the stability of peptide/HLA-A*0201 complexes. In particular, one Melan-A peptide analogue was more efficient in the generation of Melan-A peptide-specific and melanoma-reactive CTL than its parental peptide in vitr...

Considerable evidence suggests that IL-10 may have a role in the manifestation of atopic disease. We sought to test the hypothesis that at the single cell level, allergen-specific T cells have diminished IL-10 production capacity in severely affected atopics compared with asymptomatic atopics. We defined three A*0201-restricted Der p 1 CD8(+) T cell epitopes. Using human leukocyte antigen-A*020...

This paper argues that automated knowledge acquisition for diagnosis has had limited success in both failure-driven diagnosis and model-based diagnosis. The paper describes fault-based and model-based reasoning for diagnosis and surveys some of the approaches to knowledge acquisition in both areas. The Hakeem1 algorithm I am currently implementing for the automated generation of behavioural mod...

Binding of the T cell receptor (TCR) to its cognate, peptide antigen-loaded major histocompatibility complex (pMHC) is a key interaction for triggering activation and ultimately elimination target cell. Despite importance this cellular immunity, comprehensive molecular understanding TCR specificity affinity lacking. We conducted hydrogen/deuterium exchange mass spectrometry (HDX-MS) analyses in...

Fifty-nine tumor-infiltrating lymphocyte (TIL) cultures established from melanoma-invaded lymph nodes were screened for recognition of 28 melanoma-associated antigens (MAA) in association with31 HLA molecules. Twenty-three (39%) TIL lines reacted to at least one melanoma antigen. Melanosomal proteins were recognized by 19 TIL populations and the most prominent responses against these proteins w...

Background & aim: Breast cancer is recognized as the most common type of cancer among Iranian women. Cancer screening and early diagnosis are among the most effective strategies to reduce the associated mortality and healthcare costs. Accordingly, the aim of this study was to investigate the level of knowledge and practice of breast cancer screening among women referred to hospitals affiliated ...