BACKGROUND In healing wounds, some monocytes enter the wound and differentiate into fibroblast-like cells called fibrocytes. Since Toll-like receptors (TLRs) are present on monocytes, and pathogens that can infect a wound have and/or release TLR agonists, we examined whether TLR agonists affect fibrocyte differentiation. RESULTS When human peripheral blood mononuclear cells (PBMCs) were cultu...

background: adult stem cells (asc) are undifferentiated cells found throughout the body. these cells are promising tools for cell replacement therapy in neurodegenerative disease. adipose tissue is the most abundant and accessible source of asc. this study was conducted to evaluate effect of selegiline on differentiation of adipose-derived stem cells (adsc) into functional neuron-like cells (nl...

Rationale: Nuclear factor erythroid 2-related factor (Nrf)3, a member of the cap ‘N’ collar family of transcription factors that bind to the DNA-antioxidant responsive elements, is involved in reactive oxygen species balancing and in muscle precursor migration during early embryo development. Objective: To investigate the functional role of Nrf3 in smooth muscle cell (SMC) differentiation in vi...

background: vhl (von hippel-lindau), runx-3 (runt-related transcription factor 3), e-cadherin (epithelial cadherin), p15 (ink4a, cyclin dependent kinase inhibitor), and p16 (ink4b) genes are essential in hematopoiesis. the aim of this study was to explore the correlation between gene expression and promoter methylation in cd34+ stem cells before and after differentiation to erythroid lineage. m...

Tissue specific transcription factors play important roles during cellular differentiation. To regulate cellular differentiation, we paid attention to tissue specific basic helix-loop-helix (bHLH) transcription factors. In this study, we tried to regulate expression of tissue specific transcription factor for induction of cellular differentiation. To regulate expression of transcription factor,...

The in vitro process of chondrogenic differentiation of mesenchymal stem cells for tissue engineering has been shown to require three-dimensional culture along with the addition of differentiation factors to the culture medium. In general, this leads to a phenotype lacking some of the cardinal features of native articular chondrocytes and their extracellular matrix. The factors used vary, but r...

RATIONALE Nuclear factor erythroid 2-related factor (Nrf)3, a member of the cap 'N' collar family of transcription factors that bind to the DNA-antioxidant responsive elements, is involved in reactive oxygen species balancing and in muscle precursor migration during early embryo development. OBJECTIVE To investigate the functional role of Nrf3 in smooth muscle cell (SMC) differentiation in vi...