Abstract Catalytic valorization of raw glycerol derived from biodiesel into high-value chemicals has attracted great attention. Here, we report chemoenzymatic cascade reactions that convert to lactic acid and glycolic acid. In the enzymatic step, a coenzyme recycling system was developed 1,3-dihydroxyacetone (DHA) with yield 92.3% in potassium phosphate buffer (300 mM, pH 7.1) containing 100 mM...