We report kinetic studies on the reaction of a latex agglutination immunoassay used to quantify phenytoin in serum. In this assay, polystyrene particles with a covalently attached analog of phenytoin react with an antiphenytoin monoclonal antibody to form light-scattering aggregates, with the rate of this reaction being decreased by addition of phenytoin from sample. In the absence of free (sam...

In order to demonstrate that cells of Candida spp. may show considerable nonspecific agglutination in latex agglutination tests, 150 clinical and reference isolates of 12 yeast species were systematically studied by applying various test parameters. In fact, 40 (26.7%) of these isolates revealed nonspecific results, significantly associated with the time allowed for agglutination.

Hemagglutination inhibition, enzyme-linked immunosorbent assay (ELISA), and passive latex agglutination were used to test 495 human serum samples for determination of rubella immunity. Overall agreements for immune status were as follows: hemagglutination inhibition versus ELISA, 94.7% (469 of 495); hemagglutination inhibition versus passive latex agglutination (1:10 dilution), 99.2% (491 of 49...

two hundred and forty patients, who had undergone vasectomy from 1977 to 1985 and subsequent vasovasostomy ,were studied for the presence of sperm-specific antibodies by using the kibrick's gelatin agglutination test. the number of successful pregnancies and the presence of agglutination were also considered in this survey. sixty-nine pregnancies occurred in total and agglutination was present ...

background: visceral leishmaniasis (vl) is an endemic disease in some parts of iran. many techniques have been used for diagnosis of vl, among which the urine based la-tex agglutination test (katex) is a promising one. objective: to compare three diag-nostic tests of vl including katex, elisa and direct agglutination test (dat) in vl patients and healthy controls in the south west of iran. meth...

Latex agglutination reagents are widely used in microbial diagnosis, identification and serotyping. Streptococcus pneumoniae (the pneumococcus) is a major cause of morbidity and mortality world-wide. Current vaccines target the pneumococcal capsule, and there are over 90 capsular serotypes. Serotyping pneumococcal isolates is therefore important for assessing the impact of vaccination programs ...

This study compared a slide agglutination test (Denka Seiken, Tokyo, Japan) to the "gold standard" quellung reaction (Pneumotest; Statens Serum Institut, Copenhagen, Denmark) for the serogrouping of pneumococci. Two hundred clinical isolates of Streptococcus pneumoniae were used for the comparison. Each assay was performed according to the manufacturer's instructions. There was an overall agree...

the aim of the present study was to evaluate the presence anti- e. coli, -rotavirus and -coronavirus, in calves' sera. a total of 184 calves under 1 month of age (84 diarrheic & 100 normal) was studied. serological tests including: direct elisa for detection of anti-k99 e. coli, - rotavirus and - coronavirus and tube agglutination test for detection anti-o157 e. coli, antibodies were used....