Specific phenol compounds including rhododendrol (RD), a skin-brightening ingredient in cosmetics, are reported to induce leukoderma, inducing a social problem, and the elucidation of mechanism of leukoderma is strongly demanded. This study investigated the relationship among the cytotoxicities of six phenol compounds on B16F10 melanoma cells and HaCaT keratinocytes and generated reactive oxyge...

Molecular dynamics simulation of the Michaelis complex, phospho-enzyme intermediate, and the wild-type and C12S mutant have been carried out to examine hydrogen-bonding interactions in the active site of the bovine low molecular weight protein-tyrosine phosphatase (BPTP). It was found that the Sγ atom of the nucleophilic residue Cys-12 is ideally located at a position opposite from the phenylph...

: world population has been continuously raised and therefore due to this fact new source of foods, particularly proteins, are in demand. in recent years, single cell protein has been considered as an accepted substitute for animal and plant proteins. in this study, single cell protein production was studied in a batch submerged culture using aspergillus niger ptcc5012. experimental design was ...

The C-8 position of deoxyguanosine (dGuo) was hydroxylated by ascorbic acid in the presence of oxygen (O2) in 0.1 M phosphate buffer (pH 6.8) at 37 degrees C. Addition of hydrogen peroxide (H2O2) remarkably enhanced this hydroxylation. The Udenfriend system [ascorbic acid, FeII, ethylenediaminetetraacetic acid (EDTA) and O2] was also effective for hydroxylation of dGuo in high yield. Guanine re...

Reaction 1 occurring with reaction 2 involves carbon dioxide fixation. Koepsell & Johnson (1942) obtained a cell-free extract from Clo8tridium butylicum which converts pyruvic acid into acetic acid, carbon dioxide and hydrogen, and requires phosphate for the reaction. Later Koepsell, Johnson & Meek (1944) found that inorganic phosphate is taken up and labile phosphate appears. The overall react...

This study was designed to develop a sensitive and rapid method for the quantitation of risedronate in human urine using ultra-performance liquid chromatography with ultra-violet detector (UPLC-UV) and to compare bioavailability parameter of 5, 35 and 150 mg risedronate. The mobile phase consisted of sodium phosphate buffer, 1 mM etidronate-acetonitrile (95:5, v/v), pH 9.0, and was pumped at a ...

Gadoxetate disodium is a widely used magnetic resonance (MR) contrast agent for liver MR imaging, and it provides both dynamic and hepatobiliary phase images. However, acquiring optimal arterial phase images at liver MR using gadoxetate disodium is more challenging than using conventional extracellular MR contrast agent because of the small volume administered, the gadolinium content of the age...

By SANFORD LEIKIN AND SAMUEL P. BESSMAN D ISODIUM ETHYLEXEDIAMINE TETRAACETATE (EDTA) has beets known for some time to prolong coagulation in vitro.’3 During the course of treatment of seven patiemits for lead ititoxication with disodium calcium EDTA, we noted mio hemorrhagic mamiifestations.4’ However, Wishimisky6 described hypoprothrombimsemia appearing its a patiemit treated with disodium ca...

An efficient and eco-friendly protocol for the preparation of 2-amino-4H-chromenes employing a multi-component, one-pot condensation reaction between aromatic aldehydes, malononitrile and1-naphthol has been developed. The reaction of 1-naphthol with malononitrile and various aromatic aldehydes was carried out i...

1. Mitochondria were isolated from developing endosperm of Ricinus communis and were fractionated into outer membrane and inner membrane. The relative purity of the two membrane fractions was determined by marker enzymes. The fractions were also examined by negative-stain electron microscopy. 2. Membrane fractions were sequentially extracted in the following way. (a) Suspension in 0.5M-potassiu...