A comparative study on the water quality of interstitial two creeks in Rivers State was conducted. Temperature, pH, Conductivity, Dissolved oxygen, biochemical oxygen demand, Salinity, and Total dissolved solid were checked with an in-situ hand-held multi-meter (The EZODO Multi-meter). (DO) measured a Milwaukee meter while Biochemical Oxygen Demand (BOD) determined by 5-day BOD test (APHA, 2005...

This paper presents a simple, high resolution imaging approach utilizing ratiometric planar optode for simultaneous measurement of dissolved oxygen (DO) and pH. The planar optode comprises a plastic optical film coated with oxygen indicator Platinum(II) octaethylporphyrin (PtOEP) and reference quantum dots (QDs) embedded in polystyrene (PS), pH indicator 5-Hexadecanoylamino-fluorescein (5-Fluor...

Plant genetic transformation requires appropriate choice of antibiotics for regeneration of transformed tissues and elimination of Agrobacterium, and minimal concentration of selective agents for the selection of putative transformants during transformation. This study was conducted to examine the effects of three antibiotics (carbenicillin, cefotaxime, and Clavamox) on in vitro plant regenerat...

A procedure for rapid clonal propagation of Passiflora edulis Sims. f. flavicarpa Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL(-1) 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21 ± 1.13) primordial from the n...

l-Methionine γ-lyase (MGL), a pyridoxal 5'-phosphate-dependent enzyme, possesses anti-tumor activity. However, the low activity of MGL blocks the anti-tumor effect. This study describes an efficient production process for the recombinant MGL (rMGL) from Idiomarina constructed using the overexpression plasmid in Escherichia coli BL21 (DE3), purification, and large-scale production. The enzyme pr...

Although, several pineapple micropropagation protocols have already been published, significant improvement could be achieved, if the stages of in vitro culture were better defined. Our work concerned several experiments aiming at the mass production of high quality plantlets. Tissue culture experiments were therefore conducted to develop rapid multiplication procedures for Ananas comosus L. (M...

The present study was carried out to study the effect of different dosages of β-glucan suspension on immune response and disease resistance in Anabas testudineus spawns against a fungal pathogen Saprolegnia parasitica. Eight day old spawns were exposed for 3 h in four different dosages of β-glucan suspension in phosphate buffered saline at the rate of 0, 5, 10, 15 mg l(-1). The cell suspension ...