نتایج جستجو برای: dls method differential centrifugation

تعداد نتایج: 1878749  

Journal: :The Journal of Experimental Medicine 1944
W. M. Stanley

The concentration and purification of influenza virus by means of differential centrifugation in a vacuum type centrifuge, by adsorption on and elution from adult chicken red cells, by elution of the precipitate formed on freezing and thawing of allantoic fluid, by adsorption on and elution from embryonic chick red cells, and by combinations of the first method with each of the three succeeding...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2013
Lauren DePoy Rachel Daut Jonathan L Brigman Kathryn MacPherson Nicole Crowley Ozge Gunduz-Cinar Charles L Pickens Resat Cinar Lisa M Saksida George Kunos David M Lovinger Timothy J Bussey Marguerite C Camp Andrew Holmes

Drug addictions including alcoholism are characterized by degradation of executive control over behavior and increased compulsive drug seeking. These profound behavioral changes are hypothesized to involve a shift in the regulation of behavior from prefrontal cortex to dorsal striatum (DLS). Studies in rodents have shown that ethanol disrupts cognitive processes mediated by the prefrontal corte...

Journal: :Annals of clinical and laboratory science 1978
L N Sussman

The need for sophisticated component therapy has resulted in improved techniques for obtaining concentrates of platelets and granulocytes. The use of single donors as a source for these products is advisable to avoid multiple sensitizations. A method for obtaining large quantities of platelets has been established rather easily. Obtaining concentrated granulocytes represents a greater problem o...

Journal: :The Journal of Biophysical and Biochemical Cytology 1960
Edward L. Kuff Robert F. Zeigel

Prominent nucleoprotein sedimentation boundaries were demonstrable in cytoplasmic extracts of Novikoff hepatoma. Fractionation of the homogenates by differential centrifugation or a density gradient method revealed that 65 to 75 per cent of the cytoplasmic ribonucleic acid was present in the form of free ribonucleoprotein particles. After purification by differential centrifugation in dilute bu...

Objective Aspirated ovarian follicular fluids (FF) contain luteal granulosa cells (LGC) and other contaminating cell types. Several strategies such as the antibody binding methods, the flask method, the cell strainer, and the positive selection of granulosa aggregates after density gradient centrifugation, were tested as LGCs purification methods. Each of these strategies has its own advantages...

2002
Michael J. Shaw Riyaz Sikora

In this paper we propose a distributed approach to the inductive learning problem and present an implementation of the Distributed Learning System (DLS). Our method involves breaking up the data set into different sub-samples, using an inductive learning program (in our case PLS1) for each sample, and finally synthesizing the results given by each program into a final concept by using a genetic...

2014
Anees Mehdi

The standard semantics employed for description logics (DLs) adheres to the open world assumption (OWA). On one hand, such an assumption facilitates a modeler to formally represent problem domains with out explicitly expressing full information, which sometimes is even not possible. This is probably one of the main reasons of founding the Web Ontology Language (OWL) based on DLs. On the other h...

2013
Hiroyuki Yasuda Akira Matsumura Hidetomi Terai Hiromitsu Toyoda Akinobu Suzuki Sho Dohzono Kohji Tamai Hiroaki Nakamura

STUDY DESIGN A cross-sectional study. OBJECTIVE To evaluate the segmental instability of wedging segments using dynamic films and the impact of osteophyte formation on segmental motion in degenerative lumbar scoliosis (DLS). SUMMARY OF BACKGROUND DATA Evaluation of segmental instability in DLS is essential for spinal surgery to make the decision "fusion or not fusion." Some previous studies...

Journal: :Langmuir : the ACS journal of surfaces and colloids 2010
De-Hao Tsai Frank W DelRio Robert I MacCuspie Tae Joon Cho Michael R Zachariah Vincent A Hackley

Competitive adsorption kinetics between thiolated polyethylene glycol (SH-PEG) and mercaptopropionic acid (MPA) on gold nanoparticles (Au-NPs) were studied using a prototype physical characterization approach that combines dynamic light scattering (DLS) and electrospray differential mobility analysis (ES-DMA). The change in hydrodynamic particle size (intensity average) due to the formation of ...

Journal: :South African Journal of Chemical Engineering 2021

Green synthesis of silver nanoparticles (Ag NPs) utilizing plant extracts has been widely optimized these days because its eco-friendly, simple, and cost-effective manner. This present study was performed to evaluate the scale-up trial on parameter centrifugation duration Plantago major L. leaf ethanolic extract in green Ag NPs. Leaf concentration 0.25%, 70 °C temperature, 60 min time, 30 time ...

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