Yield and quality are fundamental features for any researchers during nucleic acid extraction. Here, we describe a simplified, semi-unified, effective, and toxic material free protocol for extracting DNA and RNA from different prokaryotic and eukaryotic sources exploiting the physical and chemical properties of nucleic acids. Furthermore, this protocol showed that DNA and RNA are under triple p...

DNA extracted from plants rich in polyphenols and/or polysaccharides is often problematic when subjected to polymerase chain reaction, especially when mature tissues are used for DNA extraction. In order to overcome the problems associated with poor-quality DNA extracted from such plant samples, a protocol has been developed, availing on a high salt concentration and on the combination of polyv...

An improved protocol for the isolation of high-quality DNA from fungi is described. The method involves inactivating proteins by SDS/proteinase K and precipitating polysaccharides in the presence of high salt. Further purification is based on differential solubility of DNA and high molecular weight polysaccharides in aqueous media. The procedure does not use the toxic and potentially hazardous ...

BACKGROUND With the availability of complete genomes, a systematic inventory of cellular processes becomes achievable. This requires assessing the function of all individual genes. Transfection of plasmid DNA into cell culture cells is an essential technique for this aim as it allows functional overexpression or downregulation of genes. While many robotic systems isolate plasmids for sequencing...

The aim of this study was to develop an improved procedure for preparation of chromosome suspensions, and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenization of synchronized root tips after mild fixation with formaldehyde. Histograms of relative fluorescence intensity (flow karyotypes) obtained...

Genomic DNA was isolated from leaves of three species of Jatropha, namely J. glandulifera, J. gossypifolia and J. curcas. The objective of our study was to use alcohol as a fixing solution, making liquid nitrogen unnecessary for isolation of genomic DNA from Jatropha species. The spectral quality of genomic DNA isolated using this method as measured by the A260/A280 absorbance ratio ranged from...

Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods--namely three DNA isolation protocols and three commercial DNA isolation kits--...