Interpretation of crime stain profiles is one the challenging tasks forensic scientists. Before using probabilistic genotyping system, scientists should interpret analytical and stochastic thresholds. According to guidelines presented by Scientific Working Group on DNA Analysis Methods, these thresholds shall be based supported applicable internal validation studies. In this study, we performed...

The present study was to construct a parentage verification system for Iranian Caspian horse. A total number of 45 Caspian horse samples including 14 foals for parentage verification, 17 stallion and 14 mare for individual identification were genotyped. Genomic DNA was extracted from whole blood and the genotype were analysed by PCR procedure and using 7 microsatellite markers (AHT04, HMS03, HM...

A second-generation signal amplification, nucleic acid-based test for the rapid detection and typing of herpes simplex virus (HSV) DNA was developed and evaluated with artificial and clinical specimens. The analytical sensitivity of the Hybrid Capture II (HC II) HSV DNA assay was determined by testing either cloned HSV DNA or total genomic HSV DNA titrations and resulted in detection thresholds...

conclusions the outcome of this study indicates a higher discriminatory power of the rflp analysis based on the spa gene compared to the coa gene. moreover, the results of our study reveal that the resistance rate of s. aureus to some antimicrobial agents including linezolid is a growing concern. background antibiotic resistant staphylococcus aureus is a serious public health problem worldwide....

The ability to determine nucleic acid sequences is one of the most important platforms for the detailed study of biological systems. Pyrosequencing technology is a relatively novel DNA sequencing technique with multifaceted unique characteristics, adjustable to different strategies, formats and instrumentations. The aims of this thesis were to improve the chemistry of the Pyrosequencing techniq...

Short Tandem Repeat (STR) typing plays an important role in forensic science. Currently, many laboratories return the residual unextracted DNA evidence sample to police stations according regulations Japan. However, it has not been clarified how well results of original analysis can be reproduced case retest trace or mixed from remaining samples. In this study, simulated touch samples were prep...