Over the past decade, Penetrating Keratoplasty (PKP) graft failure has been increasingly managed by Descemet Membrane Endothelial (DMEK). Moreover, Anterior Segment Optical Coherence Tomography (AS-OCT) is becoming as essential modality for perioperative evaluation. Our aim to share important surgical considerations and modifications based on preoperative evaluation using AS-OCT and, consequent...

Purpose: To compare the effect of 20% sulfur hexafluoride (SF6) with that of air on graft detachment rates for intraocular tamponade in Descemet membrane endothelial keratoplasty (DMEK). Methods: Forty-two eyes of patients who underwent DMEK by a single surgeon (A.S.J.) at Wilmer Eye Institute between January 2012 and 2014 were identified; 21 received air for intraocular tamponade and the nex...

PURPOSE To evaluate the use of a femtosecond laser combined with a microkeratome in the preparation of posterior corneal disks for Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS This experimental study involved ultrathin DSAEK tissue preparation of 22 donor corneas unsuitable for transplantation. The first cut was performed with an Intralase® FS60 laser and the second ...

PURPOSE To report a case of non-Descemet Stripping Automated Endothelial Keratoplasty (nDSAEK) using heads-up surgery. CASE/INTERVENTION The case was a 72-years-old man who had history of left eye blunt trauma since childhood. One year ago, the patient was diagnosed to have left posttraumatic bullous keratopathy. The patient underwent lt nDSAEK by using the heads-up three-dimensional (3D) sys...

The biological membrane has historically never played a very dominant role in biochemistry. It has merely been conceived as a barrier that hinds the cellinteriors from di using. In Hodgkin and Huxley 's model of nerve signaling it works as a capaciter. In this thesis I examine the e ect of the peripheral protein cytochrome c on a membrane consisting of the charged lipid DMPG. I will show that t...