PURPOSE To identify differentially expressed genes in keratoconus (KC) corneal fibroblasts. METHODS Stromal keratocytes (having a fibroblast morphology) from KC keratoplasty specimens and eye bank donor corneas were isolated and expanded using a serum containing medium. RNA was isolated from three KC fibroblast cultures and five eye bank donor cornea fibroblast cultures. The targets from the ...

The lens plays an important role in the development of the optic cup. Using the zebrafish as a model organism, questions regarding lens development can be addressed. The zebrafish is useful for genetic studies due to several advantageous characteristics, including small size, high fecundity, short lifecycle, and ease of care. Lens development occurs rapidly in zebrafish. By 72 hpf, the zebrafis...

PURPOSE To determine the immunogenic characterization of amniotic epithelium (AE), by examining the fate of allogeneic AE grafts heterotopically transplanted in the eye. METHODS Intact AE from enhanced green fluorescence protein (EGFP) transgenic mice (C57BL/6 background) and wild-type C57BL/6 mice were transplanted onto cornea or conjunctiva, or inserted into the anterior chamber (AC) of nor...

An antiserum against the nitric oxide (NO) synthetase labelled varicosities surrounding circadian pacemaker cells (“basal retinal neurons”, BRNs) in the eye of the marine mollusc Bulla gouldiana. These profiles appear to arise from extrinsic neurons with axons entering the eye via the optic nerve and are distinct from FMRFamide containing profiles, which represent the only efferent optic fibres...

Logistic regression methods were used to analyse the influence of donor and storage factors on the quality of c~meas stored by organ culture in the Bristol eve bank in 1991. Averaae donor a(18 was 57 vears fsd 22. n=2770) -and time from death to en~cleation v& 8 hours (&I 7, n&782): Corneas were placed into organ culture within 27 houra (sd 10, n+2550) of donor death and were stored for 22 days...

PURPOSE To compare the interval between death and enucleation (ΔT-O-E), between enucleation and preservation (ΔT-E-P) and the quality of the cornea before and after the implantation of new technique and sanitary rules. METHODS A retrospective study that evaluated the records of cornea donors in Sao Paulo's Santa Casa Eye Tissue Bank 2 years before and 2 years after the implementation new sani...

We report the electrochemistry, spectroscopy, and electrogenerated chemiluminescence (ECL) of donor-acceptor compounds containing a quinoxaline derivative as the central core coupled with fluorene (CFPQ, CFPP, and MFPQ) and triphenylamine moieties (MAPQ). Cyclic voltammetry of all four derivatives show reversible reduction waves (assigned to the formation of the radical anion) localized in the ...

closure of donor site of the flap has special problems. reduction of this site will decrease the morbidity of operation. in this study, we present our experience in donor site size reduction. methods between 2006 and 2008, 15 patients with skin and soft tissue defects underwent operation. in all patients, coverage of defect was performed with various flaps. substratum horizontal mat-tress sutur...

background: hepatitis b is the most common cause of acute viral hepatitis in the world. a small number of epidemiological studies, show the role of sucking insects in the transmission of hepatitis b as mechanical vector .in order to determine the prevalence of hbsag in patients with malaria and to compare with healthy blood donors, this study was conducted. methods: in this case-control study, ...

abstract mutations have been described in all of the four open reading frames of the hepatitis b virus (hbv), however, from a clinical perspective the surface escape mutant is the most troublesome. hepatitis b surface antigen (hbsag) variants may impair diagnosis, or allow the virus to escape vaccine-induced immunity or passive immunoglobulin therapy. hbv mutants with amino acid substitutions, ...