Target-derived NGF promotes the phenotypic maintenance of mature dorsal root ganglion (DRG) nociceptive neurons. Here, we provide in vivo and in vitro evidence for the presence within DRG neurons of endosomes containing NGF, activated TrkA, and signaling proteins of the Rap1/Erk1/2, p38MAPK, and PI3K/Akt pathways. Signaling endosomes were shown to be retrogradely transported in the isolated sci...

We investigated the effects of chronic compression (CCD) of the L3 and L4 dorsal root ganglion (DRG) on pain behavior in the mouse and on the electrophysiological properties of the small-diameter neuronal cell bodies in the intact ganglion. CCD is a model of human radicular pain produced by intraforaminal stenosis and other disorders affecting the DRG, spinal nerve, or root. On days 1, 3, 5, an...

To identify and analyse precursor cells of neuronal and glial cell lineages during the early development of the chick peripheral nervous system, monoclonal antibodies were raised against a population of undifferentiated cells of E6 dorsal root ganglia (DRG). Non-neuronal cells of E6 DRG express surface antigens that are recognized by four monoclonal antibodies, G1, G2, GLI 1 and GLI 2. The prop...

In the immune system, mCD24, the mouse homolog of the human glycosyl phosphatidylinositol-anchored glycoprotein CD24, may play a role in cell adhesion. In the nervous system, the function of mCD24 has not been determined, but its transient expression by neurons suggests that it may be involved in axon growth in development. Here we show that retinal ganglion cells (RGCs) and dorsal root ganglio...

Cell surface carbohydrates are thought to play important roles in the development and differentiation of mammalian cells. In previous studies we have found that one population of dorsal root ganglion (DRG) neurons is specified by the expression of complex globoseries oligosaccharides (Dodd, J., D. Solter, and T. M. Jessell (1984) Nature 311: 469-472; Jessell, T. M., and J. Dodd (1985) Philos. T...

The proneural transcription factor neurogenin 1 (neurog1) has been shown to be a key regulator of dorsal root ganglion (DRG) neuron development. Here we use a novel transgenic zebrafish line to demonstrate that the neural crest population that gives rise to DRG neurons becomes fate restricted to a neuronal/glial precursor before the onset of neurog1 function. We generated a stable transgenic ze...

BACKGROUND Abnormal acute pain after burn injury still torments patients severely. In this study, we investigated that one voltage gated sodium channel Nav1.7 plays a vital role in lowering heat pain threshold after burn injury, and the hypothesis that knockdown of Nav1.7 attenuates pain following burn injury. METHODS Sixty eight adult male Sprague-Dawley rats were divided into 4 treatment gr...

Voltage-gated sodium channels interact with cytosolic proteins that regulate channel trafficking and/or modulate the biophysical properties of the channels. Na(v)1.6 is heavily expressed at the nodes of Ranvier along adult CNS and PNS axons and along unmyelinated fibers in the PNS. In an initial yeast two-hybrid screen using the C terminus of Na(v)1.6 as a bait, we identified FHF2B, a member of...

Atopic dermatitis is a chronic inflammatory skin disease, the pathology of which characterized by barrier dysfunction, microbiome shift, immune dysregulation initiated severe itching sensation. Because its pathophysiological and clinical significance, research on understanding mechanism itch finding new therapeutic targets it has been highlighted recently. To dissect cellular/molecular changes ...