A total of 10 cyanogen bromide peptides were isolated from the S-beta-carboxymethyl iron protein of nitrogenase. Purification of these peptides was performed mainly by gel filtration on Sephadex G-50; by ascending paper chromatography using the solvent system of pyridine, isoamyl alcohol, 0.1 M ammonium hydroxide; and also, in some cases, with additional steps such as anion exchange column chro...

In order to obtain a highly concentrated, pure and super-hot [ƒÁ-32P]ATP, we improved the purification method of super-hot [ƒÁ-32P]ATP which was synthesized by the method of Johnson and Walseth (1979). The super-hot [ƒÁ-32P]ATP was synthesized in a relatively large volume (2 ml) of reaction mixture and purified using semi-micro scale anion exchange chromatography (Dowex 1•~2, 60-70ƒÊl column vo...

Glycerinated rabbit psoas fibers were tested for their ability to contract under the influence of creatine phosphate and creatine kinase in the absence of free nucleotide. Tension development by the fibers was observed upon addition of creatine phosphate to the medium containing creatine kinase purified to the first lyophilization stage. However, when the enzyme was washed free of nucleotides b...

Quinaldic acid, which was found to be a metabolite of kynurenic acid (l), was not conjugated by the rabbit (2), but was excreted by the rat both free and as the glycine conjugate (3). When quinaldic acid was administered orally or parenterally to the cat, the urine contained little, if any, quinaldic acid or quinaldylglycine, but about two-thirds of the compound was accounted for as quinaldylgl...

During a study of the effect of 2,4-dichlorophenoxyacetic acid on the soluble nucleotides in soybean seedlings (1)) a nonnucleotide compound accumulated in the treated seedlings. The same compound was found in high concentrations in immature soybean seeds that would not germinate (2). The compound inhibited seed germination and markedly affected respiration and phosphorylation of both intact so...

The removal of dissolved organic carbon (DOC) is a key factor in the control disinfection by-product (DBP) formation drinking water. Ion exchange process deals with naturally negatively charged matter. A fluidized bed column test has been chosen to parallel efficiency 4 different anionic resins (Purolite PPA860S, Dowex TAN-1, Amberlite IRA-958 and IRA-410) terms DOC fraction related DBP reducti...

After online experiments with Hf (t1/2 = 76 s, decaying into Lu) at GSI [1] we performed two experiments with the multi-column-technique [2] with Rutherfordium. Rf was produced in the Cm(O, 5n) reaction at the PSI Philips Cyclotron. A 730 μg/cm Cm target was bombarded with a 0.5 μApart O beam. The KCl aerosols transporting the activities was continuously dissolved in a degasser with a yield > 8...