BACKGROUND AND OBJECTIVES Quantitative real time PCR (qPCR) offers rapid diagnosis of rickettsial infections. Thus, successful treatment could be initiated to avoid unfavorable outcome. Our aim was to compare two qPCR assays for Rickettsia detection and to evaluate their contribution in early diagnosis of rickettsial infection in Tunisian patients. PATIENTS AND METHODS Included patients were ...

A truly universal nucleobase enables a host of novel applications such as simplified templates for PCR primers, randomized sequencing and DNA based devices. A universal base must pair indiscriminately to each of the canonical bases with little or preferably no destabilization of the overall duplex. In reality, many candidates either destabilize the duplex or do not base pair indiscriminatingly....

INTRODUCTION: Molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as the leishmaniases. The present study describes the optimization and evaluation of a duplex real-time quantitative PCR (qPCR) protocol developed for the simultaneous detection of Leishmania infantum DNA and sample quality control. METHODS: After ...

Next-generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of ~1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely proble...

Abstract Background The prevalence of gastrointestinal (GI) neoplastic polyps in Jack Russell terriers (JRTs) has increased Japan since the late 2000s. Recently, we demonstrated that JRTs with GI harbor identical germline variant APC gene (c.[462_463delinsTT]) heterozygous state. Thus, this disease is an autosomal dominant hereditary disorder. Although affected have distinct features, such as d...

The acquisition of channel state information (CSI) in Frequency Division Duplex (FDD) massive MIMO has been a formidable challenge. In this paper, we address problem with novel CSI feedback framework enabled by the partial reciprocity uplink and downlink channels wideband regime. We first derive closed-form expression rank covariance matrix for given angle-delay distribution. A low-rankness pro...