The aim of this study was to investigate the effects of erythropoietin (EPO) on the impairment of autophagy induced by lipopolysaccharide (LPS) in primary cultured rat glomerular mesangial cells (GMCs). Rat GMCs were isolated and cultured in normal glucose, high-glucose, LPS or LPS + EPO medium. At 24 and 72 h of culture, the cells were examined for expression levels of the autophagy markers LC...

A comparison was made of the relative efficiencies of three enrichment media, RapidChek Escherichia coli O157:H7 enrichment broth (REB), R&F broth (RFB), and modified E. coli broth containing novobiocin (mEC+n), and four selective plating media for detection of cold- and freeze-stressed E. coli O157:H7 in raw ground beef. Ground beef (25 g) was inoculated with E. coli O157:H7 at < or =0.5 and <...

As an artificial intelligence method, machine learning (ML) has been widely used in prediction models of high-dimensional datasets. This study proposes ML the Gradient Boosted Regression Tree (GBRT), to predict intensity changes tropical cyclones (TCs) Western North Pacific at 12-, 24-, 36-, 48-, 60-, and 72-h (hr) forecasting lead time model is optimized by Bayesian Optimization algorithm. The...

Insektisida adalah jenis pestisida untuk memberantas serangga. Nikotin senyawa alkaloid utama dalam daun tembakau yang aktif sebagai insektisida. pada penelitian ini diperoleh dari hasil ekstraksi limbah puntung rokok. Tujuan mempelajari pengaruh variabel waktu dan volume pelarut sampel digunakan, menentukan rendemen densitas nikotin terhadap presentase kematian serangga, mengetahui kandungan i...

The effect of fasting for 12,36 and 72 h was studied in twenty-nine healthy subjects (seventeen women and twelve men). Measurements were made of cardiovascular variables, metabolic rate, respiratory exchange ratio, plasma metabolites, insulin, thyroid hormones and catecholamines. During starvation there were no significant changes in blood pressure, whilst heart rate (beatslmin) increased at 36...

Three experiments were conducted to determine the relationship between concentrations of insulin-like growth factor-I (IGF-I) in ovarian follicular fluid and various biochemical markers of follicular differentiation in bovine follicles. In Experiment I, ovaries were removed on Days 7, 14, 28, 42, or 56 after parturition from a total of 21 cows. In Experiment II, ovaries of 31 cows were removed ...

hematopoietic cord blood (cb) stem cell transplantation has more advantages to other cell sources because of lower graft versus host disease (gvhd). interleukin-15(il-15) is an immunoregulatory cytokine, known to enhance cytolytic function of cord natural killer (nk) cells. the aim of this study was to investigate the effect of il-15 on nk cytotoxicity simultaneously in different cell death sta...

The present study is concerned with the improvement of Bacillus amyloliquefaciens strain UNG-16 for alpha amylase production. The bacterial culture was exposed to UV irradiation at 1.6×10 J/m/S for 15-60 min. However, UV induced viables did not give improved alpha amylase production; therefore chemical mutation using ethyl methane sulphonate (EMS 50-300 μl/ml) was undertaken for 10-60 min. The ...

1 The research was sponsored by the Department for Communities and Local Government, UK. It was led jointly by the Local Governance Research Unit at De Montfort University, UK (Lawrence Pratchett) and the e-Democracy Centre at the University of Geneva, Switzerland (Uwe Seurdult and Fernando Mendez). Country reports were developed by specialist country analysts: Estonia Liia Hanni (e-Governance ...

A semi-micro method for determination of plasma renin activity (PRA) by radioimmunoassay of generated angiotensin I (AI) is described. The method permits measurement of PRA on 250 mul of plasma without loss of specificity, sensitivity, accuracy or preision. The small sample size has considerable application in terms of investigation of infants and young children. A reference range was establish...