background and objectives: urinary tract infection (uti) is one of the most common infections in human, and uropathogenic escherichia coli (upec) strains are the most common cause of uti. these strains colonize in bladder and cause cystitis, which could cause pyelonephritis by movement to the kidneys. since fimh protein has a very important role in colonization of upec strains and causing infec...

background: it seems that the success of vaccination for cancer immunotherapy such as dendritic cell (dc) based cancer vaccine is hindered through a powerful network of immune system suppressive elements in which regulatory t cell is the common factor. foxp3 transcription factor is the most specific marker of regulatory t cells. in different studies, targeting an immune response against regulat...

Translocation, processing and secretion of YvaY, a Bacillus subtilis protein of unknown function, were characterised both in B. subtilis and in Escherichia coli. In its natural host B. subtilis, YvaY was transiently synthesised at the end of the exponential growth phase. It was efficiently secreted into the culture supernatant in spite of a calculated membrane spanning domain in the mature part...

conclusions full factorial design experimental approach helped to identify the critical culture condition parameters in heterologous expression of v2r. it would allow studying the parameters affecting the function of this protein in future experiments. results the maximum amount of protein production was obtained by the addition of 0.75 mm iptg at 37°c after three hours. the most important fact...

The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant production. Continuous culture the most common method grow cells for In study, K12 strain of E. was as production protein. LeExp1 gene plant. Since related found very small amounts plants, it has been reproduced PCR and made workable with this method. T17 vector (...

The ability to utilize Escherichia coli as a heterologous system in which to study the regulation of Agrobacterium tumefaciens virulence genes and the mechanism of transfer DNA (T-DNA) transfer would provide an important tool to our understanding and manipulation of these processes. We have previously reported that the rpoA gene encoding the alpha subunit of RNA polymerase is required for the e...

The ability of staphylococcal protein A (SPA) to bind to the Fc part of IgG has been used for the purification of a number of heterologous gene products as fusion proteins. Both the SPA promoter and signal sequence function in Escherichia coli, as well as in a number of Gram-positive bacteria, which facilitates comparisons of the expressed specific products in different hosts. The expression sy...

Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system.  Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing...

protein a is a commercially important protein in biotechnological and medicinal applications. the great value of this protein and its applications in genetic and protein engineering and microbial researches as well as the growing use in biochemical industries, biotechnology, medicine and pharmacology, highlight the importance of the present study. in this survey the encoding genes of full-lengt...