After fertilization, rapid changes of the Caenorhabditis elegans cytoskeleton occur in the transition from meiosis to mitosis, requiring precise regulation. The MEI-1/MEI-2 katanin microtubule-severing complex is essential for meiotic spindle formation but must be quickly inactivated to allow for proper formation of the mitotic spindle. MEI-1/MEI-2 inactivation is dependent on multiple redundan...

Ubiquitination, the crucial posttranslational modification that regulates the eukaryotic proteome, is carried out by a trio of enzymes, known as E1 [ubiquitin (Ub)-activating enzyme], E2 (Ub-conjugating enzyme), and E3 (Ub ligase). Although most E2s can work with any of the three mechanistically distinct classes of E3s, the E2 UBCH7 is unable to function with really interesting new gene (RING)-...

Human papilloma viruses (HPVs) are involved not only in anogenital but also in head and neck carcinogenesis. So far almost 100 sub-types of HPV have been identified but HPVs 16, 18 and 31 are most common high risk types in oro-pharyngeal cancers. Since HPVs are involved in cancers, therefore, preventive and therapeutic options will reduce the prevalence, mortality and morbidity associated with ...

•TRIP12 facilitates PROTAC-induced and CRL2VHL-mediated degradation of BRD4•TRIP12 specifically assembles K29-linked Ub chains•TRIP12 CRL2VHL cooperatively assemble K29/K48-branched chains on BRD4•Targeted uses unique mechanisms rather than a simple “hijack” Targeted protein is an emerging therapeutic paradigm. Small-molecule degraders such as proteolysis-targeting chimeras (PROTACs) induce the...

Covalent attachment of one or more ubiquitin molecules to protein substrates governs numerous eukaryotic cellular processes including apoptosis, cell division and immune response. Ubiquitylated proteins can be targeted for degradation but ubiquitylation also mediates processes such as protein-protein interactions and cell signalling, depending on the type of ubiquitin conjugation. Ubiquitin lig...