To determine the elution pattern and electrophoretic characterization of granular-secretory fraction of the bovine fetal cotyledons, an experimental procedure was developed. A multi-proteic complex extract of the granular-secretory fraction of the bovine fetal cotyledons, was frozen at -20◦C, then was centrifuged at 46,000 x g for 60 min at 4 ◦C. After that, the supernatant was removed and save...

The extraction behavior of thymus and the composition of fractions prepared from this organ has been studied. Sequential extraction methods using 0.15 M NaCl followed by water gave information with respect to the weight fraction of cytoplasmic and nuclear constituents. Lipide, nucleic acid, and electrophoretic analysis of the extracts provided additional information. A less complex electrophore...

Some years ago, Theobald Smith and his associates (1) showed that passive immunity acquired by the new-born calf from colostrum is of prime importance in protection against infectious disease. At about the same time, Orcutt and Howe (2) observed that, after the ingestion of colostrum, agglutinins appear in the calf serum associated with a globulin which is precipitable at low concentrations of ...

The objective of this study was to compare the electrophoretic pattern serum proteins in newborn calves, lambs, goat kids and piglets precolostral blood samples. Blood samples were collected within 30 min birth, prior ingestion first colostrum, concentrations total protein fractions analyzed using electrophoresis on agarose gel. size shape electrophoretograms differed among investigated animal ...

The evaluation of different methods for kafirin extraction was made to obtain techno-functional concentrates from two sorghum cultivars. were rheologically evaluated in a “dough-like” resin system assess their potential as gluten substitute. Under acidic extractions (ethanol 70%, pH 2.5), yields 56.98% and 46.31% white (WS) red (RS) obtained, with the highest purity WS (74.54%). A characteristi...

It is a well established fact that the circulating proteins of the human are profoundly affected by surgical, accidental and combat trauma. The medical literature on this topic emphasizes the remarkable quantitative and qualitative changes in intracellular and extracellular protein constituents occurring after and in response to injury. Fundamental information concerning these metabolic alterat...

The electrophoretic pattern of the large microtubule-associated protein, MAP2, changes during rat brain development. Immunoblots of NaDodSO4 extracts obtained from the cerebral cortex, cerebellum, and thalamus at 10-15 days after birth reveal only a single electrophoretic species when probed with any of three MAP2 monoclonal antibodies. By contrast, adult MAP2 contains two immunoreactive specie...

The effects of high pressure (to 600 MPa) at different temperatures (20 to 60 °C) for 20 min on protein solubilization and electrophoretic pattern in beef post-rigor longissimus dorsi muscle were studied. The results showed that protein solubilization increased with increasing temperature, especially from 40 °C to 60 °C. A regular trend of protein solubilization was found when isolated myofibri...

Peripheral blood cell preparation from 23 normal subjects and 72 patients with acute and 32 patients with chronic myeloid leukemia were cultured in vitro and released plasminogen activators were analyzed. The quantity of plasminogen activator secreted by leukemic cells varied widely and could not be correlated with the clinical severity of the disease. Immunochemical and electrophoretic techniq...

An electrophoretic variant of lactate dehydrogenase (LD) M(A) subunit was discovered in a female patient with chest pain. Her LD activity in serum was within the normal reference interval, and analysis of her LD isoenzyme pattern showed an abnormal migration indicating a fast-type LD-M(A) subunit variant. DNA analysis of the mutant LD-M gene detected a single base substitution, an A to G transi...