A novel 10 kDa protein with anti-HIV-1 reverse transcriptase (RT) inhibitory activity was isolated from leaves of Canna indica L. using a combination of native-PAGE and ammonium sulfate precipitation. HIV-1 and RT inhibitory activity was measured using a syncytium forming (deltaTat/Rev) MC99 virus in Tat/Rev transfected 1A2 cell line and ELISA technique, respectively. Edman N-terminal and inter...

The aim of the study was to detect HIV RNA in seropositive patients using RT-PCR method and thus, to establish PCR methodology in the routine laboratory works. The total of 33 examined persons were divided in two groups: 1) 13 persons seropositive for HIV; and 2) 20 healthy persons - randomly selected blood donors that made the case control group. The subjects age was between 25 and 52 years (a...

Passion fruit plants are self-incompatible and fruiting is exclusively dependent on cross-pollination, which in nature performed by carpenter (mamangava) bees, especially Xylocopa spp. (family Apidae). To ensure satisfactory productivity, daily manual pollination a common practice among passionflower growers. Therefore, we studied whether mechanically transmits the cowpea aphid-borne mosaic vir...

The paper was conducted to assess the importance and challenges encountered in the current machine evaluation system in an electronics company as perceived by the respondents. In addition, it also sought to understand the acceptability of the new developed Real-Time Auto-locked Software for Electronics Company (RT-ASEC). The new automated machine evaluation software can detect any irregular mac...