Sum mary A n enzyme-linked immunosorbent assay ( ELISA) for sulphones in urine is described . This method for monitoring dapsone ( D D S) self­ administration is comp ared with the quantitative d etermination of DDS/ creatinine (D/C) rat io s, currently used . Urine samples collected from 1 0 volunteers o n 1 4 consecutive days after taking single doses of 1 00 mg D D S are studied , together w...

The aim of this study was to detect the amount of aflatoxin M1 (AFM1) in pasteurized milk samples in Mashad in northeast of Iran. For this purpose, 42 milk samples were collected from retail stores during fall 2011 and analyzed for AFM1 by enzyme-linked immunosorbent assay (ELISA) technique. All the analyses were done twice. Results showed presence of AFM1 in 97.6% of the examined milk samples ...

A sensitive method for detecting rubella IgM antibody, the first step of which is the attachment of the serum IgM to a solid phase, is described. Specific IgM antibody was found in all 52 people with acquired rubella in the early convalescent period, in all 38 infants with congenital rubella examined in the first seven months of life, and in 23 of 26 people immunised one to four months before t...

BACKGROUND alpha,alpha-Trehalase, located on renal proximal tubules, is a glycoprotein that hydrolyses alpha,alpha-trehalose to two glucose molecules. Urinary trehalase reflects damage to renal proximal tubules, but its activity has not been measured routinely because measurement of catalytic activity is rather complicated and because conventional assays for enzyme activity might not reflect al...

An enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgM antibodies against Mycoplasma pneumoniae, performed with commercial antigen and reagents, is compared with the complement fixation test (CF) in a serological study of 209 human sera. Concordant results were usually obtained by CF test and by IgG ELISA in sera from patients with recent M pneumoniae infection. In contra...

BACKGROUND Chronic obstructive pulmonary disease (COPD) is an inflammatory pulmonary disorder with systemic inflammatory manifestations that are mediated by circulating acute-phase reactants. This study compared an enzyme-linked immunosorbent assay (ELISA) to a nephelometric technique for the measurement of serum C-reactive protein (CRP) and serum amyloid A (SAA) and investigated how the choice...

A time resolved fluorometric immunoassay (TRFIA) has been developed and compared with an in house enzyme linked immunosorbent assay (ELISA) and commercial ELISA (Bindazyme) for the detection of tetanus antitoxin in human sera. A panel of 132 sera submitted for routine testing was used. Scatterplots showed a high degree of correlation between all three assays, although some divergence of results...

BACKGROUND A logical progression of the widely used microtiter plate ELISA is toward a protein array format that allows simultaneous detection of multiple analytes at multiple array addresses within a single well. Here we describe the construction and use of such a multiplex ELISA to measure prostate-specific antigen (PSA), alpha1-antichymotrypsin-bound PSA (PSA-ACT), and interleukin-6 (IL-6). ...

AIM To introduce and validate the new method of diagnosing ovine and caprine brucellosis in a rapid, accurate and inexpensive manner by using i-ELISA (serum/milk) technique. METHODS Serum and milk samples from brucella RB and CFT negative (n=881) and positive (n=755) animals were used. Standardization of tests was through the Bommeli ELISA-BESW (Brucella Bang) standard and our Institute's (MK...