نتایج جستجو برای: embryo donor
تعداد نتایج: 133294 فیلتر نتایج به سال:
To determine if blastocyst trophectoderm biopsy for PGT-A is associated with an increased rate of live birth per embryo in good prognosis IVF patients at a single center. We performed retrospective cohort study undergoing transfer cycles center from 1/1/2017 through 12/31/2019. evaluated the transferred and without two groups patients: using embryos donor oocytes autologous less than 35 years o...
The mixing of mitochondrial DNA (mtDNA) from the donor cell and the recipient oocyte in embryos and offspring derived from somatic cell nuclear transfer (SCNT) compromises genetic integrity and affects embryo development. We set out to generate SCNT embryos that inherited their mtDNA from the recipient oocyte only, as is the case following natural conception. While SCNT blastocysts produced fro...
The transplantation of somatic cell nuclei to enucleated eggs has shown that genes can be reprogrammed to an embryonic pattern of expression, thereby indicating a reversal of their epigenetic state. However, in Xenopus nuclear transfer experiments using both endoderm and neuroectoderm donor cells, we have observed substantial overexpression of donor cell type-specific genes, both spatially and ...
Fanconi anemia is a rare autosomal recessive disease characterized by bone marrow failure, developmental anomalies, and a high incidence of myelodysplasia and acute myeloid leukemia. Stem cell transplantation is the only curative treatment. In the absence of matched- sibling donor, an alternative mismatched family or matched unrelated donor can be used, but the results are inferior to the match...
Dolly the sheep cloning “the first cloning process” was derived by somatic cell nuclear transfer and it had reported that the clone got premature senescence as a result of continues telomere shortening and ongoing decrease in telomerase activity by time; thus majority of recent researches is directed to use other types of donor cells to be transferred into the recipient enucleated oocyte to mai...
Repeated oocyte collection by transvaginal ultrasound-guided follicular puncture (Ovum Pick-Up: OPU), implicitly associated to in vitro embryo production (IVEP), has become alternative and competitive to superovulation for embryo production in cattle. It is alternative because it can be applied successfully irrespective of the reproductive status of the donor, i.e. in pregnant and acyclic anima...
Incomplete epigenetic reprogramming of donor cell nuclei is one of the main contributors to the low efficiency of somatic cell nuclear transfer (SCNT). To improve the success of SCNT, somatic cell DNA methylation levels must be reduced to those levels found in totipotent embryonic cells. Recent studies have demonstrated that miR-148a can affect DNA methylation via DNMT1 modulation in various ca...
The mechanism of transfection by Rous sarcoma virus DNA was investigated by assaying DNA-mediated transformation under conditions which restricted secondary virus infection. Chicken embryo fibroblasts which were genetically resistant to virus infection as a result of the absence of receptors for virus penetration were also resistant to transformation by integrated or unintegrated Rous sarcoma v...
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