one of the important methods of plant propagation in large scale so as genetic manipulation and artificial seed production is somatic embryogenesis technique. the obtained results from this methods related to different factors including condition of culture medium. therefore, the aim of this study conduction were determining the role of molybdenum during carrot petiole somatic embryogenesis. at...

the aim of the study was to compare the parthenogenetic activation and in vitro fertilization (ivf) of in vitro matured caprine oocytes. a total of 881 cumulus-oocyte complexes (coc’s) were collected from 243 ovaries. oocytes were matured in tcm-199 medium containing ecg (20 iu/ml), hcg (20 iuμg/ml), oestradiol-17β (1 μg/ml), bsa embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...

AIM OF THE STUDY Pilot study to analyse the efficacy and embryo morphology using a new human embryo culture medium (GM501) versus the conventional used medium (ISM1). METHODS Over a four-month period, all patients at the Leuven Institute of Fertility and Embryology (LIFE) were -randomly allocated to have their embryos cultured in either the standard sequential culture medium ISM1 (control) or...

parsi zira, bunium persicum (boiss.) b. fedtsch., or black zira, a member of the umbellifera family, is one of the most important medicinal species with high economic value. generally, there is limited information about in vitro culture of bunium persicum. in this study, the effect of various media (ms, b5) and combinations of plant growth regulators, naa and 2,4-d alone or together with kin, o...

background: to achieve multiple oocytes for in vitro fertilization, ovulation induction is induced by gonadotropins however, it has several effects on oocytes and embryo quality and endometrium receptivity. the aim of this study was to assess ultrastructural changes of corpus luteum after ovarian induction using human menopausal gonadotropin (hmg) and human chorionic gonadotropin (hcg) during l...

Successful efficient whole cauliflower plant regeneration via somatic embryogenesis from root derived callus tissue was achieved. The research confirmed for the first time the capability of mass production of cauliflower somatic embryos through the indirect pathway. The best callus induction and proliferation was on semi solid Murashige and Skoog (MS) medium supplemented with 2, 4-D at 0.15 mg ...

Somatic embryogenesis was initiated in C. sativus L. from shoot meristems on LS medium containing BAP (2×10-5 M)+NAA (2×10-5 M). Various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. Somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. Matured embryos coul...

Hybrids as various resources have valuable importance in tree breeding. Today, improvement programs by using poplar and willows and their hybrids play basic role in supplying wood and decreasing pressure on natural forests. This research was carried out in order to study inter-generic hybridization possibility between Salix aegyptica and Populus caspica species from Salicaceae to achieve new hy...